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AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level
- Source :
- STAR Protocols, Vol 1, Iss 2, Pp 100092-(2020), STAR Protoc
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Summary By including oligonucleotide-labeled antibodies into high-throughput single-cell RNA-sequencing protocols, combined transcript and protein expression data can be acquired on the single-cell level. Here, we describe a protocol for the combined analysis of over 40 proteins and 400 genes on over 104 cells using the nano-well based Rhapsody platform. We also include a workflow for sample multiplexing, which uniquely identifies the initial source of cells (such as tissue type or donor) in the downstream analysis after upstream pooling. For complete information on the use and execution of this protocol, please refer to Mair et al. (2020) .
- Subjects :
- Proteomics
General Immunology and Microbiology
Sequence Analysis, RNA
Computer science
Gene Expression Profiling
General Neuroscience
Gene Expression
High-Throughput Nucleotide Sequencing
Proteins
Computational biology
Cellular level
Multiplexing
Article
Antibodies
General Biochemistry, Genetics and Molecular Biology
Workflow
Tissue type
Upstream (networking)
Single-Cell Analysis
Transcriptome
lcsh:Science (General)
Protocol (object-oriented programming)
Gene
Gene transcript
lcsh:Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 26661667
- Volume :
- 1
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- STAR Protocols
- Accession number :
- edsair.doi.dedup.....5f8aef7647f03be0b1d106172ce17dd5