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Accurate Determination of S-Phase Fraction in Proliferative Cells by Dual Fluorescence and Peroxidase Immunohistochemistry with 5-Bromo-2′-Deoxyuridine (BrdU) and Ki67 Antibodies
- Publication Year :
- 2011
- Publisher :
- SAGE Publications, 2011.
-
Abstract
- To ensure the maintenance of tissues in mammals, cell loss must be balanced with cell production, the proliferative activity being different from tissue to tissue. In this article, the authors propose a new method for the quantification of the proliferative activity, defined as the S-phase fraction of actively cycling cells, by dual labeling with fluorescence and peroxidase immunohistochemistry using BrdU (marker of S-phase) and Ki67 antibodies (marker of G1-, S-, G2-, and M-phases) after a one-step antigen retrieval. In the generative cell zones of fundic and pyloric glandular stomachs, where the majority of cells were cycling, the authors measured a proliferative activity of 31%. In the epithelium of the forestomach and the skin, where cycling cells are intermingled with G0 and differentiated cells, proliferative activities were 21% and 13%, respectively. In the adrenal cortex, in which cycling cells were sparsely distributed, the proliferative activity reached 32%. During the regenerative process in the skin after a lesion, the proliferative activity increased in proximity to the wound. The present one-step dual-labeling method has revealed that the proliferative activity is different between tissues and depends on the physiological or pathological state.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Histology
Cellular differentiation
Biology
Article
Antibodies
S Phase
chemistry.chemical_compound
Mice
Adrenal Glands
medicine
Gastric mucosa
Animals
Cell Proliferation
Skin
Wound Healing
Staining and Labeling
Cell growth
Stomach
Molecular biology
Immunohistochemistry
Epithelium
Mice, Inbred C57BL
medicine.anatomical_structure
Ki-67 Antigen
Antigen retrieval
chemistry
Bromodeoxyuridine
Gastric Mucosa
Indicators and Reagents
Anatomy
Wound healing
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....5fad950fb983df0503ac8807106b19ee