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Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern
- Source :
- Kyei-Baafour, E, Oppong, M, Kusi, K A, Frempong, A F, Aculley, B, Arthur, F K N, Tiendrebeogo, R W, Singh, S K, Theisen, M, Kweku, M, Adu, B, Hviid, L & Ofori, M F 2021, ' Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern ', PLoS ONE, vol. 16, no. 4, e0249936 . https://doi.org/10.1371/journal.pone.0249936, PLoS ONE, PLoS ONE, Vol 16, Iss 4, p e0249936 (2021)
- Publication Year :
- 2021
-
Abstract
- Detection of antibody reactivity to appropriate, specific parasite antigens may constitute a sensitive and cost-effective alternative to current tools to monitor malaria transmission across different endemicity settings. This study aimed to determine the suitability of IgG responses to a number of P. falciparum antigens as markers of transmission intensity and pattern. Antibody responses to multiple malaria antigens were determined in 905 participants aged 1–12 years from three districts with low (Keta), medium (Hohoe) and high (Krachi) transmission intensity in the Volta region of Ghana. Blood film microscopy slides and dry blood spots (DBS) were obtained for parasitaemia detection and antibody measurement, respectively. Sera were eluted from DBS and levels of IgG specific for 10 malaria antigens determined by a multiplex assay. Results were compared within and among the districts. Total IgG responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, and PfRh2a and PfRh2b were higher in Krachi than in Hohoe and Keta. Seroprevalence of IgG specific for MSPDBLLeucine, RON4, and PfRh2b were also highest in Krachi. Responses to RALP-1, PfRh2a and PfRh2b were associated with patent but asymptomatic parasitaemia in Keta, while responses to MSPDBL1, MSPDBLLeucine, MSP2-FC27, RAMA, Rh2-2030, and PfRh2b were associated with parasite carriage in Hohoe, but not in Krachi. Using ROC analysis, only PfRh2b was found to predict patent, but asymptomatic, parasitaemia in Keta and Hohoe. Antibody breadth correlated positively with age (r = 0.29, pPfRh2b has potential as a marker of malaria transmission intensity and pattern. This could have implications for malaria control programs and vaccine trials.
- Subjects :
- Male
Plasmodium
Physiology
Antibody Response
Antibodies, Protozoan
Biochemistry
Ghana
law.invention
Geographical Locations
Medical Conditions
Seroepidemiologic Studies
law
Immune Physiology
Medicine and Health Sciences
Parasite hosting
Enzyme-Linked Immunoassays
Malaria, Falciparum
Child
Immune Response
Protozoans
Immune System Proteins
Multidisciplinary
biology
Malarial Parasites
Eukaryota
Transmission (mechanics)
Child, Preschool
Medicine
Female
Antibody
medicine.symptom
Research Article
Science
Immunology
Plasmodium falciparum
Antigens, Protozoan
Research and Analysis Methods
Asymptomatic
Antibodies
Antigen
Parasite Groups
parasitic diseases
Parasitic Diseases
medicine
Humans
Seroprevalence
Immunoassays
Organisms
Biology and Life Sciences
Proteins
Infant
Tropical Diseases
biology.organism_classification
medicine.disease
Parasitic Protozoans
Malaria
Cross-Sectional Studies
ROC Curve
Immunoglobulin G
People and Places
Africa
Immunologic Techniques
biology.protein
Parasitology
Apicomplexa
Biomarkers
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Kyei-Baafour, E, Oppong, M, Kusi, K A, Frempong, A F, Aculley, B, Arthur, F K N, Tiendrebeogo, R W, Singh, S K, Theisen, M, Kweku, M, Adu, B, Hviid, L & Ofori, M F 2021, ' Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern ', PLoS ONE, vol. 16, no. 4, e0249936 . https://doi.org/10.1371/journal.pone.0249936, PLoS ONE, PLoS ONE, Vol 16, Iss 4, p e0249936 (2021)
- Accession number :
- edsair.doi.dedup.....5fb9b59fa843fdea4023ac744831fec0