Back to Search
Start Over
Molecular surveillance of resistance to pyrethroids insecticides in Colombian Aedes aegypti populations
- Source :
- PLoS Neglected Tropical Diseases, Vol 15, Iss 12, p e0010001 (2021), PLoS Neglected Tropical Diseases
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Introduction In Colombia, organochloride, organophosphate, carbamate, and pyrethroid insecticides are broadly used to control Aedes aegypti populations. However, Colombian mosquito populations have shown variability in their susceptibility profiles to these insecticides, with some expressing high resistance levels. Materials and methods In this study, we analyzed the susceptibility status of ten Colombian field populations of Ae. aegypti to two pyrethroids; permethrin (type-I pyrethroid) and lambda-cyhalothrin (type-II pyrethroid). In addition, we evaluated if mosquitoes pressured with increasing lambda-cyhalothrin concentrations during some filial generations exhibited altered allelic frequency of these kdr mutations and the activity levels of some metabolic enzymes. Results Mosquitoes from all field populations showed resistance to lambda-cyhalothrin and permethrin. We found that resistance profiles could only be partially explained by kdr mutations and altered enzymatic activities such as esterases and mixed-function oxidases, indicating that other yet unknown mechanisms could be involved. The molecular and biochemical analyses of the most pyrethroid-resistant mosquito population (Acacías) indicated that kdr mutations and altered metabolic enzyme activity are involved in the resistance phenotype expression. Conclusions In this context, we propose genetic surveillance of the mosquito populations to monitor the emergence of resistance as an excellent initiative to improve mosquito-borne disease control measures.<br />Author summary The main method of preventing Aedes-borne diseases such as dengue, Zika, and chikungunya is by targeting the primary mosquito vector, Aedes aegypti, with insecticides. However, the success of these vector control strategies is jeopardized by the widespread development of insecticide resistance in mosquito populations. Furthermore, the molecular mechanisms of insecticide resistance in Ae. aegypti are still not well understood, resulting in limited resistance mitigation and management strategies. In this paper, we found that resistance to some pyrethroid insecticides in different Colombian cities is associated with three allelic substitutions V419L, V1016I, and F1534C, on the voltage-gated sodium channel gene, known as kdr (‘knock-down resistance’) mutations, with all three mutations present in mosquitoes resistant to pyrethroids. The data also showed that kdr mutations are important in conferring low resistance levels, but after around 10-fold intensity, the allele frequencies don’t change, indicating that other mechanisms contribute to the resistance. Thus, we found that mosquitoes under selective pressure with insecticides present also altered enzymatic activities such as esterases and mixed-function oxidases, indicating that kdr mutations and metabolic enzymes are involved in the resistance expression. The findings on the extent of insecticide resistance and the molecular mechanisms underpinning the problem will impact the surveillance, selection, and rational use of insecticides by local health authorities.
- Subjects :
- Insecticides
Life Cycles
Heredity
Arthropoda
RC955-962
Variant Genotypes
Disease Vectors
Aedes Aegypti
Colombia
Mosquitoes
Homozygosity
Insecticide Resistance
Medical Conditions
Larvae
Aedes
Arctic medicine. Tropical medicine
Nitriles
Pyrethrins
parasitic diseases
Medicine and Health Sciences
Genetics
Animals
Point Mutation
Permethrin
Organisms
Public Health, Environmental and Occupational Health
Biology and Life Sciences
Eukaryota
Agriculture
Invertebrates
Insect Vectors
Insects
Species Interactions
Genetic Mapping
Infectious Diseases
Mutation
Insect Proteins
Public aspects of medicine
RA1-1270
Agrochemicals
Zoology
Entomology
Research Article
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 15
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....5fbb7e718673ce6c4794cb0cd6db4a5e