Additional file 1: Fig. S1. of An Assessment of Fixed and Native Chromatin Preparation Methods to Study Histone Post-Translational Modifications at a Whole Genome Scale in Skeletal Muscle Tissue

Setup of chromatin preparation conditions. Table S1. ChIP-qPCR primers. Table S2. Quantity of chromatin and DNA used throughout the protocol. Fig. S2. Quality assessment of the sequencing reads. Fig. S3. Boxplot representing the distribution of the 40 M peaks lengths. Fig. S4. X-ChIP-seq and N-ChIP-seq peaks analysis. Table S3. Broad peak detection analysis by MACS2 for both H3K27me3 X-ChIP-seq and N-ChIP-seq experiments. Fig. S5. Comparison of H3K27me3-enriched peak regions detected by MACS2 and between MACS2 and epic. Supplemental methods X-ChIP-seq protocol for hypothalamus tissue samples. Table S4. Sequencing results of hypothalamus X-ChIP-seq analyzed using epic. Fig. S6. Fragmentation assessment of chromatin before immunoprecipitation for hypothalamus samples and X-ChIP results at three control loci. (PDF 1487 kb)