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Establishment of complement-resistant retroviral vector by homologous restriction factor 20 gene
- Source :
- Gene Therapy. 5:282-285
- Publication Year :
- 1998
- Publisher :
- Springer Science and Business Media LLC, 1998.
-
Abstract
- Homologous restriction factor 20 (HRF20, CD59) is one of the complement regulatory factors. In this study, the complement-resistant retroviral vector, which possesses the HRF20 gene as a selection gene, was constructed and examined. The virus-producing cell, transduced with complement-resistant retroviral vector, was established after complement-dependent selection. NIH3T3 and PK15 cells transduced with this virus-producing cell were suc- cessfully selected by complement-dependent selection, which showed significant expression of the HRF20 antigen. In addition, these cells, transduced with tissue plasminogen activator (tPA) cDNA using complement-resistant retroviral vector, expressed tPA antigen after complement-dependent selection. These findings suggest that complement-resistant retroviral vector can be used for the double transduction of HRF20, as well as other genes.
- Subjects :
- Genetic Vectors
CD59 Antigens
Enzyme-Linked Immunosorbent Assay
CD59
Biology
Cell Line
Viral vector
Mice
Transduction (genetics)
Complementary DNA
Gene expression
Genetics
Animals
Humans
Molecular Biology
Gene
Complement Inactivator Proteins
T-plasminogen activator
Genetic transfer
Gene Transfer Techniques
3T3 Cells
Blood Proteins
Molecular biology
Retroviridae
Tissue Plasminogen Activator
Molecular Medicine
Carrier Proteins
Subjects
Details
- ISSN :
- 14765462 and 09697128
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Gene Therapy
- Accession number :
- edsair.doi.dedup.....5ff6d4cc6b6b5b889120c1936926a617