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Oral delivery of water-soluble compounds to the phytoseiid mite Neoseiulus californicus (Acari: Phytoseiidae)
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 10, p e0223929 (2019)
- Publication Year :
- 2019
- Publisher :
- Public Library of Science, 2019.
-
Abstract
- Phytoseiids are predatory mites that prey on other mites and small arthropods, and several species are used in commercial agriculture for biological control of pests. To optimize phytoseiid mites' use in biocontrol, an efficient method for oral delivery of test compounds is required to assess their sensitivities to pesticides, RNAi for gene functional analysis and artificial diets. Here we developed four methods for oral delivery of a solution of xenobiotics to different life stages of the commercially available generalist predatory mite Neoseiulus californicus: (i) soaking mites in the solution, or allowing them to feed on (ii) spider mites soaked in the solution, (iii) a solution droplet, or (iv) solution-saturated filter paper. As measured by ingestion of a tracer dye, the droplet-based feeding system was most efficient; the dye was observed in the alimentary canal of >90% test mites of all life stages, with no mortality. The droplet-based feeding system was also effective for the commercially available specialist predatory mite Phytoseiulus persimilis, with >80% delivery efficiency. This study paves the way for development of methods for high-throughput RNAi and for toxicological or nutritional assays in phytoseiid mites.
- Subjects :
- 0106 biological sciences
Life Cycles
Biological pest control
01 natural sciences
Biochemistry
Predation
Toxicology
Larvae
RNA interference
Filter Paper
immune system diseases
Group-Specific Staining
Acari
Staining
Larva
Mites
Fluids
Multidisciplinary
biology
integumentary system
Physics
Eukaryota
Agriculture
Laboratory Equipment
Nucleic acids
Genetic interference
Physical Sciences
Medicine
Engineering and Technology
Epigenetics
Female
Research Article
Phytoseiidae
States of Matter
Arthropoda
Science
Equipment
Tracer Dye Staining
Research and Analysis Methods
010603 evolutionary biology
Xenobiotics
Gene Delivery
parasitic diseases
Mite
Genetics
Gene Expression and Vector Techniques
Animals
Pesticides
Molecular Biology Techniques
Pest Control, Biological
Molecular Biology
Acaridae
Molecular Biology Assays and Analysis Techniques
business.industry
Pest control
Organisms
Biology and Life Sciences
Liquids
Pesticide
biology.organism_classification
Invertebrates
respiratory tract diseases
High-Throughput Screening Assays
010602 entomology
Solubility
Specimen Preparation and Treatment
Predatory Behavior
RNA
Gene expression
Pest Control
business
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....603b5071540b90a6109ae99eb9003e86