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Mitogenic response of near-diploid mouse cell line m5S/1M induced by epidermal growth factor
- Source :
- Journal of Cellular Physiology. 142:99-107
- Publication Year :
- 1990
- Publisher :
- Wiley, 1990.
-
Abstract
- A nonmalignant near-diploid cell line m5s/1M, established by Sasaki and Kodama (J. Cell. Res., 131:114-122, 1987), was shown to respond to the epidermal growth factor (EGF). The m5s/1M cells showed high sensitivity to post-confluence inhibition of cell division and formed a uniform monolayer after the cells had become confluent. The addition of EGF resulted in loss of contact-dependent inhibition of growth and caused a massive piling up of a multilayered array of cells after they had become confluent. When EGF was removed from the medium, the cell number decreased rapidly, and the cells formed a uniform monolayer at the density observed in the absence of EGF. m5S/1M cells have high- and low-affinity receptors for EGF (approximately 40,000 receptors per cell), and the apparent dissociation constants of the EGF-binding reactions were 3.3 nM and 0.15 nM, respectively. The effect of EGF on the intracellular mobilization of Ca2+ and the formation of inositol phosphates was studied by using the calcium-sensitive fluorescent indicator fura 2 and [3H]inositol. EGF had no effect either on the mobilization of cytosolic free calcium [( Ca2+]i) or on the formation of inositol phosphates in m5s/1M cells, whereas bradykinin induced a rapid increase in both [Ca2+]i and inositol phosphates. Analysis of the glycosphingolipid (GSL) composition of m5S/1M cells showed that globotriaosylceramide (Gb3Cer), which is known to be a Burkitt lymphoma-associated antigen, is specifically expressed in the EGF-treated cells. The expression of Gb3Cer is dependent on the presence of EGF, with a reversible shift in GSL composition being observed in the presence or absence of EGF.
- Subjects :
- Cell division
Fura-2
Physiology
Inositol Phosphates
Clinical Biochemistry
Mitosis
Palmitic Acids
Biology
Glycosphingolipids
Cell Line
Mice
chemistry.chemical_compound
Epidermal growth factor
Animals
Inositol
Carbon Radioisotopes
Receptor
Epidermal Growth Factor
Globosides
Cell growth
Trihexosylceramides
Cell Biology
Fibroblasts
Diploidy
Molecular biology
chemistry
Cell culture
Calcium
Cell Division
hormones, hormone substitutes, and hormone antagonists
Intracellular
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 142
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....603d576f499fe2605320335db72f5991