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Identification of Target Genes of Antiarrhythmic Traditional Chinese Medicine Wenxin Keli
- Source :
- Cardiovascular Therapeutics, Cardiovascular Therapeutics, Vol 2020 (2020)
- Publication Year :
- 2020
- Publisher :
- Hindawi, 2020.
-
Abstract
- Wenxin Keli (WXKL) is a traditional Chinese medicine drug approved for the treatment of cardiovascular diseases. This study aimed to identify WXKL-targeting genes involved in antiarrhythmic efficacy of WXKL. The Traditional Chinese Medicine Systems Pharmacology (TCMSP) technology platform was used to screen active compounds of WXKL and WXKL-targeting arrhythmia-related genes. A pig model of myocardial ischemia (MI) was established by balloon-expanding the endothelium of the left coronary artery. Pigs were divided into the model group and WXKL group (n = 6). MI, QT interval, heart rate, and arrhythmia were recorded, and the mRNA expression of target genes in myocardial tissues was detected by PCR. Eleven active ingredients of WXKL and eight WXKL-targeting arrhythmia-related genes were screened. Five pathways were enriched, and an “ingredient-gene-path” network was constructed. WXKL markedly decreased the incidence of arrhythmia in the MI pig model (P<0.05). The QT interval was significantly shortened, and the heart rate was slowed down in the WXKL group compared with the model group (P<0.05). In addition, the expression of sodium channel protein type 5 subunit alpha (SCN5A) and beta-2 adrenergic receptor (ADRB2) was downregulated, while muscarinic acetylcholine receptor M2 (CHRM2) was upregulated in the WXKL group (P<0.05). In conclusion, WXKL may shorten the QT interval and slow down the heart rate by downregulating SCN5A and ADRB2 and upregulating CHRM2 during MI. These findings provide novel insight into molecular mechanisms of WXKL in reducing the incidence of ventricular arrhythmia.
- Subjects :
- 0301 basic medicine
Male
Time Factors
Endothelium
Article Subject
Swine
Myocardial Ischemia
Adrenergic
Alpha (ethology)
Action Potentials
RM1-950
Traditional Chinese medicine
030204 cardiovascular system & hematology
Pharmacology
QT interval
NAV1.5 Voltage-Gated Sodium Channel
03 medical and health sciences
0302 clinical medicine
Heart Rate
Heart rate
medicine
Diseases of the circulatory (Cardiovascular) system
Animals
Pharmacology (medical)
Gene Regulatory Networks
Protein Interaction Maps
Medicine, Chinese Traditional
Receptor
Receptor, Muscarinic M2
business.industry
Muscarinic acetylcholine receptor M2
Arrhythmias, Cardiac
General Medicine
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
RC666-701
Swine, Miniature
Therapeutics. Pharmacology
Receptors, Adrenergic, beta-2
Cardiology and Cardiovascular Medicine
business
Anti-Arrhythmia Agents
Research Article
Drugs, Chinese Herbal
Subjects
Details
- Language :
- English
- ISSN :
- 17555914
- Database :
- OpenAIRE
- Journal :
- Cardiovascular Therapeutics
- Accession number :
- edsair.doi.dedup.....6064eb08e628cc7a0af84a50de095251
- Full Text :
- https://doi.org/10.1155/2020/3480276