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Development of an LC-MS/MS method for the detection of traces of peanut allergens in chili pepper
- Source :
- Analytical and Bioanalytical Chemistry. 409:5201-5207
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- The recent detection of nuts (including peanut) in spices across the globe has led to enormous recalls of several spices and food products in the last two years. The lack of validated detection methods specific for spices makes it difficult to assess allergen presence at trace levels. Because of the urgent need for confirmation of possible peanut presence in chili peppers, an LC-MS/MS method was optimized and developed for this particular food matrix. Although several studies optimized LC-MS detection strategies specific for peanuts, the presence of complex components in the spices (e.g., phenolic components) makes method optimization and validation necessarily. Focus was laid on validation of the method with real incurred chili peppers (whereby a known amount of peanut is added) at low concentrations, to deal with possible matrix interferences. LC-MS/MS proves to be a good alternative to the currently most applied methods (ELISA and RT-PCR) and can be used as a complementary method of analysis when results are unclear. Peanut marker peptides were selected based on their abundancy in digested incurred chili peppers. The limit of detection was determined to be 24 ppm (mg peanut/kg), a level whereby the risk for potential allergic reactions is zero, considering the typical portion size of spices. The chili pepper powder under investigation proved to contain low levels of peanuts after LC-MS/MS, ELISA, and RT-PCR testing. Graphical abstract Standard curve of the detected peanuts in chili pepper samples using the novel LC-MS/MS method.
- Subjects :
- Arachis
Chili pepper
Enzyme-Linked Immunosorbent Assay
Food Contamination
Portion size
Polymerase Chain Reaction
01 natural sciences
Biochemistry
Analytical Chemistry
Matrix (chemical analysis)
0404 agricultural biotechnology
Limit of Detection
Tandem Mass Spectrometry
Lc ms ms
Food science
Volume concentration
Detection limit
Chemistry
010401 analytical chemistry
food and beverages
04 agricultural and veterinary sciences
Method of analysis
Allergens
040401 food science
0104 chemical sciences
Food products
Capsicum
Peptides
Food Analysis
Chromatography, Liquid
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 409
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....60674cdb25fa1d89b7dafa09f46848f0