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MicroRNA-200c modulates the expression of MUC4 and MUC16 by directly targeting their coding sequences in human pancreatic cancer
- Source :
- PLoS ONE, Vol 8, Iss 10, p e73356 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Transmembrane mucins, MUC4 and MUC16 are associated with tumor progression and metastatic potential in human pancreatic adenocarcinoma. We discovered that miR-200c interacts with specific sequences within the coding sequence of MUC4 and MUC16 mRNAs, and evaluated the regulatory nature of this association. Pancreatic cancer cell lines S2.028 and T3M-4 transfected with miR-200c showed a 4.18 and 8.50 fold down regulation of MUC4 mRNA, and 4.68 and 4.82 fold down regulation of MUC16 mRNA compared to mock-transfected cells, respectively. A significant reduction of glycoprotein expression was also observed. These results indicate that miR-200c overexpression regulates MUC4 and MUC16 mucins in pancreatic cancer cells by directly targeting the mRNA coding sequence of each, resulting in reduced levels of MUC4 and MUC16 mRNA and protein. These data suggest that, in addition to regulating proteins that modulate EMT, miR-200c influences expression of cell surface mucins in pancreatic cancer.
- Subjects :
- lcsh:Medicine
Biology
Open Reading Frames
03 medical and health sciences
0302 clinical medicine
RNA interference
Cell Line, Tumor
Pancreatic cancer
Gene Order
microRNA
Gene expression
medicine
Humans
Coding region
lcsh:Science
Base Pairing
030304 developmental biology
Regulation of gene expression
0303 health sciences
Binding Sites
Multidisciplinary
Base Sequence
Mucin-4
Gene Expression Profiling
Mucin
lcsh:R
Membrane Proteins
medicine.disease
Molecular biology
Cell biology
Gene Expression Regulation, Neoplastic
Pancreatic Neoplasms
MicroRNAs
Tumor progression
CA-125 Antigen
030220 oncology & carcinogenesis
RNA Interference
lcsh:Q
sense organs
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....606e47563001b7b03748877d66b8d616