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A highly selective ATP-responsive biomimetic nanochannel based on smart copolymer
- Source :
- Analytica Chimica Acta. 1188:339167
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- ATP-sensitive potassium (KATP) channels couple intracellular metabolism to the electrical activity by regulating K+ flux across the plasma membrane, thus playing an important role in both normal and pathophysiology. To understand the mechanism of ATP regulating biological ion channels, developing an ATP-responsive artificial nanochannel is an appealing but challenging topic because KATP channel is a heteromultimer of two subunits (potassium channel subunit (Kir6.x) and sulfonylurea receptor (SUR)) and exhibit dynamic functions with adjustability and reversibility. Inspired by the structure of KATP channels, we designed a smart copolymer modified nanochannel that may address the challenge. In the tricomponent poly(N-isopropylacrylamide) (PNIPAAm, PNI)-based copolymer system, phenylthiourea was used to bind the phosphate units of nucleotides and phenylboronic acid was introduced to combine the pentose ring of the nucleoside unit. Besides, a –COOH group with electron-withdrawing property was added into the phenylthiourea units, which may promote the hydrogen-bond-donating ability of thiourea. Specially, the smart copolymer not only provided static binding sites for recognition but also translated the recognition of ATP into their dynamic conformational transitions by changing the hydrogen-bonding environments surrounding PNIPAAm chains, thus achieving the gating function of nanochannel, which resembled the integration and coordination of Kir6.x and SUR units in active KATP. The ATP-regulated ion channel exhibited excellent stability and reversibility. This study is the first example showing how to learn from nature to assemble the ATP-responsive artificial nanochannel and demonstrate the possible mechanism of ATP gating.
- Subjects :
- chemistry.chemical_classification
Binding Sites
ATP-sensitive potassium channel
Nucleotides
Chemistry
Gating
Sulfonylurea Receptors
Biochemistry
Potassium channel
Analytical Chemistry
chemistry.chemical_compound
Adenosine Triphosphate
Membrane
Biomimetics
Biophysics
Environmental Chemistry
Sulfonylurea receptor
Nucleotide
Phenylboronic acid
Spectroscopy
Ion channel
Subjects
Details
- ISSN :
- 00032670
- Volume :
- 1188
- Database :
- OpenAIRE
- Journal :
- Analytica Chimica Acta
- Accession number :
- edsair.doi.dedup.....607357b42c6e42532df1e4a3d2ed22d2