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The effect of chemical modification of Saccharomyces cerevisiae on electrophoretic mobility, cell-wall structure and amphotericin B uptake
- Source :
- Biochimica et biophysica acta. 845(2)
- Publication Year :
- 1985
-
Abstract
- Saccharomyces cerevisiae NCYC 239 suspended in solutions of NaCl showed two distinct plateaus in plots of electrophoretic mobility vs. pH, corresponding to p K a values of approx. 2 and 5. This is in contrast to cells suspended in buffer where only a single p K a (4) can be determined. Modification of cells with KI/I 2 or nitrous acid led to altered electrophoretic mobility, indicating the presence of sulphydryl and amino groups, respectively, in the yeast cell surface, whereas uranyl nitrate modification had little effect, suggesting phosphate groups to be absent. Electron micrographs showed visible effects of KI/I 2 and nitrous acid modification on cell membrane structure, and in these modified cells amphotericin B uptake was rapid. It is suggested that diffusion through the cell wall is the rate-limiting step for amphotericin B uptake. An activation energy of 20 kJ·mol −1 was determined for uptake of amphotericin B by unmodified cells.
- Subjects :
- Electrophoresis
Chemical Phenomena
Saccharomyces cerevisiae
Nitrous Acid
Cell membrane
Cell wall
chemistry.chemical_compound
Cell Wall
Amphotericin B
medicine
Freeze Fracturing
Molecular Biology
Nitrites
Nitrous acid
biology
Potassium Iodide
Chemical modification
Cell Biology
Hydrogen-Ion Concentration
Phosphate
biology.organism_classification
Yeast
Chemistry
Microscopy, Electron
medicine.anatomical_structure
chemistry
Uranyl nitrate
Biochemistry
Uranyl Nitrate
Biophysics
Thermodynamics
Iodine
Subjects
Details
- ISSN :
- 00063002
- Volume :
- 845
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.doi.dedup.....608b14386c91931eaaf5023f822a4821