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Metabolic modulations of Pseudomonas graminis in response to H2O2 in cloud water
- Source :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2019, 9, pp.12799. ⟨10.1038/s41598-019-49319-2⟩, Scientific Reports, 2019, 9, pp.12799. ⟨10.1038/s41598-019-49319-2⟩, Scientific Reports (9), . (2019), Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- In cloud water, microorganisms are exposed to very strong stresses especially related to the presence of reactive oxygen species including H2O2 and radicals, which are the driving force of cloud chemistry. In order to understand how the bacterium Pseudomonas graminis isolated from cloud water respond to this oxidative stress, it was incubated in microcosms containing a synthetic solution of cloud water in the presence or in the absence of H2O2. P. graminis metabolome was examined by LC-MS and NMR after 50 min and after 24 hours of incubation. After 50 min, the cells were metabolizing H2O2 while this compound was still present in the medium, and it was completely biodegraded after 24 hours. Cells exposed to H2O2 had a distinct metabolome as compared to unexposed cells, revealing modulations of certain metabolic pathways in response to oxidative stress. These data indicated that the regulations observed mainly involved carbohydrate, glutathione, energy, lipid, peptides and amino-acids metabolisms. When cells had detoxified H2O2 from the medium, their metabolome was not distinguishable anymore from unexposed cells, highlighting the capacity of resilience of this bacterium. This work illustrates the interactions existing between the cloud microbial metabolome and cloud chemistry.
- Subjects :
- 0301 basic medicine
Atmospheric chemistry
Microorganism
Chimie analytique
Air Microbiology
lcsh:Medicine
medicine.disease_cause
complex mixtures
Article
Mass Spectrometry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Adenosine Triphosphate
Pseudomonas
Metabolome
medicine
Metabolomics
lcsh:Science
chemistry.chemical_classification
Reactive oxygen species
Multidisciplinary
biology
lcsh:R
Humidity
Glutathione
Hydrogen Peroxide
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Metabolic pathway
Oxidative Stress
030104 developmental biology
chemistry
Biochemistry
lcsh:Q
sense organs
Microcosm
[CHIM.OTHE]Chemical Sciences/Other
Analytical chemistry
030217 neurology & neurosurgery
Bacteria
Oxidative stress
Autre (Sciences du Vivant)
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2019, 9, pp.12799. ⟨10.1038/s41598-019-49319-2⟩, Scientific Reports, 2019, 9, pp.12799. ⟨10.1038/s41598-019-49319-2⟩, Scientific Reports (9), . (2019), Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
- Accession number :
- edsair.doi.dedup.....6093a2ff33ceea4e613dfd34c1d58e8f