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A role for the VLA-4 integrin in the activation of human memory B cells
- Source :
- European Journal of Immunology. 27:2757-2764
- Publication Year :
- 1997
- Publisher :
- Wiley, 1997.
-
Abstract
- It is generally recognized that activation through membrane effector molecules such as CD40 or the B cell receptor (BCR) is mandatory to allow B cells to proliferate and differentiate into antibody (Ab)-secreting cells in response to cytokines. We show here that purified tonsillar B cells can be stimulated directly by a cytokine combination to proliferate and secrete immunoglobulins when cultures are performed at high cell density. The contact-mediated activation of B cells in this experimental system is strongly inhibited both by anti-very late antigen (VLA)-4 monoclonal Ab and by a peptide containing the LDV sequence specifically recognized by the alpha 4 integrin binding site. These reagents also significantly suppressed the B cell responses elicited by engagement of the BCR or CD40. Our data reveal that memory B cells but not virgin or germinal center B cells are sensitive to the direct stimulatory effect of cytokines in high-density cultures. Finally, we found that the dual expression of the alpha and beta chains of VLA-4 is a distinctive feature of the memory B cell population. Collectively, our findings support the notion that VLA-4-dependent homotypic B cell interactions can mediate a co-stimulatory signal to human memory B cells and might participate in the B cell activation triggered through the BCR and CD40.
- Subjects :
- Integrins
Integrin beta Chains
Immunology
B-cell receptor
Naive B cell
Receptors, Lymphocyte Homing
Immunoglobulins
Cell Count
Cell Communication
Integrin alpha4beta1
Lymphocyte Activation
Receptors, Very Late Antigen
medicine
Humans
Immunology and Allergy
CD40 Antigens
Memory B cell
Cells, Cultured
B cell
B-Lymphocytes
CD40
biology
Germinal center
Cell Differentiation
Interleukin-10
Cell biology
B-1 cell
medicine.anatomical_structure
Polyclonal B cell response
biology.protein
Interleukin-2
Immunologic Memory
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....60aaa4c28526174e5afc7e3c9b017e93