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Distinct populations of tumor-initiating cells derived from a tumor generated by rat mammary cancer stem cells
- Source :
- Proceedings of the National Academy of Sciences of the United States of America 105 (2008): 16940–16945. doi:10.1073/pnas.0808978105., info:cnr-pdr/source/autori:I Zucchi 1, S Astigiano, G Bertalot, S Sanzone, C Cocola, P Pelucchi, G Bertoli, M Stehling, O Barbieri, A Albertini, H R Schöler, B G Neel, R A Reinbold, R Dulbecco/titolo:Distinct populations of tumor-initiating cells derived from a tumor generated by rat mammary cancer stem cells/doi:10.1073%2Fpnas.0808978105./rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2008/pagina_da:16940/pagina_a:16945/intervallo_pagine:16940–16945/volume:105, Proceedings of the National Academy of Sciences of the United States of America 105 (2008): 16940–16945. doi:10.1073/pnas.0808978105, info:cnr-pdr/source/autori:Zucchi I, Astigiano S, Bertalot G, Sanzone S, Cocola C, Pelucchi P, Bertoli G, Stehling M, Barbieri O, Albertini A, Schöler HR, Neel BG, Reinbold RA, Dulbecco R./titolo:Distinct populations of tumor-initiating cells derived from a tumor generated by rat mammary cancer stem cells./doi:10.1073%2Fpnas.0808978105/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2008/pagina_da:16940/pagina_a:16945/intervallo_pagine:16940–16945/volume:105
- Publication Year :
- 2008
- Publisher :
- The Academy, Washington, D.C. , Stati Uniti d'America, 2008.
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Abstract
- Tumors derived from rat LA7 cancer stem cells (CSCs) contain a hierarchy of cells with different capacities to generate self-renewing spheres and tubules serially ex vivo and to evoke tumors in vivo . We isolated two morphologically distinct cell types with distinct tumorigenic potential from LA7-evoked tumors: cells with polygonal morphology that are characterized by expression of p21/ WAF1 and p63 and display hallmarks of CSCs and elongated epithelial cells, which generate tumors with far less heterogeneity than LA7 CSCs. Serial transplantation of elongated epithelial cells results in progressive loss of tumorigenic potential; tumor heterogeneity; CD44, E-cadherin, and epithelial cytokeratin expression and increased α-smooth muscle actin I and vimentin expression. In contrast, serial transplantation of LA7 CSCs can be performed indefinitely and results in tumors that maintain their heterogeneity, consistent with self-renewal and multilineage differentiation potential. Collectively, our data show that polygonal cells are CSCs, whereas epithelial elongated cells are lineage-committed progenitors with tumorigenic potential, and suggest that tumor progenitors, although lacking indefinite self-renewal potential, nevertheless may make a substantial contribution to tumor development. Because LA7 cells can switch between conditions that favor maintenance of pure CSCs vs. differentiation into other tumor cell types, this cell system provides the opportunity to study factors that influence CSC self-renewal and differentiation. One factor, p63 , was identified as a key gene regulating the transition between CSCs and early progenitor cells.
- Subjects :
- Cell type
Cellular differentiation
Mice, SCID
Mice
Cytokeratin
Mammary Glands, Animal
Cancer stem cell
Cell Line, Tumor
Neoplasms
Animals
Cell Lineage
Progenitor cell
Multidisciplinary
biology
tumor initiating cells
Cancer stem cells
Stem Cells
CD44
Mammary Neoplasms, Experimental
Cell Differentiation
Biological Sciences
Immunohistochemistry
Clone Cells
Rats
Disease Models, Animal
Cell culture
Immunology
Neoplastic Stem Cells
biology.protein
Cancer research
Female
Stem cell
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America 105 (2008): 16940–16945. doi:10.1073/pnas.0808978105., info:cnr-pdr/source/autori:I Zucchi 1, S Astigiano, G Bertalot, S Sanzone, C Cocola, P Pelucchi, G Bertoli, M Stehling, O Barbieri, A Albertini, H R Schöler, B G Neel, R A Reinbold, R Dulbecco/titolo:Distinct populations of tumor-initiating cells derived from a tumor generated by rat mammary cancer stem cells/doi:10.1073%2Fpnas.0808978105./rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2008/pagina_da:16940/pagina_a:16945/intervallo_pagine:16940–16945/volume:105, Proceedings of the National Academy of Sciences of the United States of America 105 (2008): 16940–16945. doi:10.1073/pnas.0808978105, info:cnr-pdr/source/autori:Zucchi I, Astigiano S, Bertalot G, Sanzone S, Cocola C, Pelucchi P, Bertoli G, Stehling M, Barbieri O, Albertini A, Schöler HR, Neel BG, Reinbold RA, Dulbecco R./titolo:Distinct populations of tumor-initiating cells derived from a tumor generated by rat mammary cancer stem cells./doi:10.1073%2Fpnas.0808978105/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2008/pagina_da:16940/pagina_a:16945/intervallo_pagine:16940–16945/volume:105
- Accession number :
- edsair.doi.dedup.....60ac5140b70fe3fd828452503a4ece60