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Novel transient outward K + current of mature murine hippocampal neurones
- Source :
- Pfl�gers Archiv European Journal of Physiology. 434:195-202
- Publication Year :
- 1997
- Publisher :
- Springer Science and Business Media LLC, 1997.
-
Abstract
- Hippocampal neurones were freshly isolated from the brain of adult mice and voltage-dependent K+ currents were recorded with the whole-cell patch-clamp technique. Three components of transient K+ current (IA) were isolated when analyzing data with exponential functions or treating neurones with a variety of voltage protocols and pharmacologic agents. Subtraction of the delayed rectifier current (I K) from the K+ currents elicited after prepulses to –120 mV of varying duration revealed fast (IAf) and slow (IAs) components with decay time constants of 45 ± 8 and 612 ± 140 ms, respectively; the corresponding time constants for the removal of inactivation were 12.3 and 189.6 ms. Both tetraethylammonium and dendrotoxin selectively inhibited IAs. 4-Aminopyridine (4-AP) specifically blocked IAf and 40% of IAs with different affinities. Therefore, the properties of a 4-AP-resistant (IAsR) and a 4-AP-sensitive (IAsS) component of IAs were compared. These data suggest that three distinct subtypes of K+ currents contribute to the IA of mature murine hippocampal neurones.
- Subjects :
- Neurons
Potassium Channels
Time Factors
Tetraethylammonium
Dose-Response Relationship, Drug
Physiology
Clinical Biochemistry
4-Aminopyridine
Time constant
Dendrotoxin
Hippocampal formation
Hippocampus
Mice
chemistry.chemical_compound
Decay time
Delayed rectifier
chemistry
Physiology (medical)
Biophysics
medicine
Animals
Receptor
Neuroscience
medicine.drug
Subjects
Details
- ISSN :
- 14322013 and 00316768
- Volume :
- 434
- Database :
- OpenAIRE
- Journal :
- Pfl�gers Archiv European Journal of Physiology
- Accession number :
- edsair.doi.dedup.....60db793ce24d4ee31dc41be1a0c4230c