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A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae
- Source :
- Genes and Development, Genes and Development, Cold Spring Harbor Laboratory Press, 2006, 20 (12), pp.1609-20. ⟨10.1101/gad.385706⟩
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- In many organisms, mitochondria import nuclear DNA-encoded small RNAs. In yeast Saccharomyces cerevisiae, one out of two cytoplasmic isoacceptor tRNAsLys is partially addressed into the organelle. Mitochondrial targeting of this tRNA was shown to depend on interaction with the precursor of mitochondrial lysyl–tRNA synthetase, preMsk1p. However, preMsk1p alone was unable to direct tRNA targeting, suggesting the existence of additional protein factor(s). Here, we identify the glycolytic enzyme, enolase, as such a factor. We demonstrate that recombinant enolase and preMSK1p are sufficient to direct tRNA import in vitro and that depletion of enolase inhibits tRNA import in vivo. Enzymatic and tRNA targeting functions of enolase appear to be independent. Three newly characterized properties of the enolase can be related to its novel function: (1) specific affinity to the imported tRNA, (2) the ability to facilitate formation of the complex between preMsk1p and the imported tRNA, and (3) partial targeting toward the mitochondrial outer membrane. We propose a model suggesting that the cell exploits mitochondrial targeting of the enolase in order to address the tRNA toward peri-mitochondrially synthesized preMsk1p. Our results indicate an alternative molecular chaperone function of glycolytic enzyme enolase in tRNA mitochondrial targeting.
- Subjects :
- MESH: Protein Transport
Saccharomyces cerevisiae Proteins
MESH: Mitochondria
Saccharomyces cerevisiae
Enolase
Mitochondrion
Models, Biological
Ribosomal Protein S6 Kinases, 90-kDa
MESH: Phosphopyruvate Hydratase
RNA Transport
03 medical and health sciences
MESH: Cation Transport Proteins
MESH: Cell Compartmentation
MESH: Saccharomyces cerevisiae Proteins
RNA, Transfer
MESH: RNA Transport
Genetics
MESH: Protein Binding
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Cation Transport Proteins
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
MESH: Ribosomal Protein S6 Kinases, 90-kDa
biology
030302 biochemistry & molecular biology
MESH: Models, Biological
RNA
biology.organism_classification
MESH: RNA, Transfer
MESH: Saccharomyces cerevisiae
Transport protein
Cell Compartmentation
Mitochondria
MESH: Solubility
Protein Transport
Enzyme
Biochemistry
chemistry
Solubility
Phosphopyruvate Hydratase
Transfer RNA
Bacterial outer membrane
Developmental Biology
Protein Binding
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 08909369
- Database :
- OpenAIRE
- Journal :
- Genes and Development, Genes and Development, Cold Spring Harbor Laboratory Press, 2006, 20 (12), pp.1609-20. ⟨10.1101/gad.385706⟩
- Accession number :
- edsair.doi.dedup.....60e095630477b2d00fae749b0b3e3f4e