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Mast Cells Contribute to Radiation-Induced Vascular Hyperpermeability
- Source :
- Radiation Research. 185:182-189
- Publication Year :
- 2016
- Publisher :
- Radiation Research Society, 2016.
-
Abstract
- Induction of vascular hyperpermeability is one of the early vascular responses to radiation exposure and is considered to contribute to subsequent fibrosis and tissue injuries. However, the mechanism underlying radiation-induced hyperpermeability has not yet been clearly elucidated. Here, we provide experimental evidence indicating that mast cells contribute to the increase in vascular permeability for albumin in normal mouse skin after irradiation. Vascular permeability in the skin of C3H mice increased after 2, 15 and 50 Gy irradiation, peaked at 24 h after irradiation and gradually decreased thereafter to the baseline level within 3-10 days. Both the extent and duration of hyperpermeability were dose dependent. We found significant degranulation of mast cells in the skin after 15 Gy irradiation. To further investigate the role of mast cells in the radiation-induced increase in vascular permeability, we measured vascular permeability in the skin of mast cell-deficient mice (WW(v)) and their wild-type littermates at 24 h after irradiation. Vascular permeability in WW(v) mice did not change, whereas that in wild-type mice significantly increased after irradiation. There were no appreciable changes in the total tissue levels of vascular endothelial growth factor or endothelial nitric oxide synthase after 15 Gy irradiation and there was no detectable expression of inducible nitric oxide synthase. Collectively, these results show that exposure to radiation induces vascular hyperpermeability in a dose-dependent manner and that mast cells contribute to this process.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Endothelium
Biophysics
Serum albumin
Vascular permeability
Radiation Dosage
Article
Permeability
Capillary Permeability
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Fibrosis
Skin Physiological Phenomena
Internal medicine
medicine
Animals
Radiology, Nuclear Medicine and imaging
Mast Cells
Serum Albumin
Skin
Mice, Inbred C3H
Radiation
biology
Degranulation
Dose-Response Relationship, Radiation
medicine.disease
Nitric oxide synthase
Vascular endothelial growth factor
Dose–response relationship
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
030220 oncology & carcinogenesis
Immunology
biology.protein
Endothelium, Vascular
Subjects
Details
- ISSN :
- 19385404 and 00337587
- Volume :
- 185
- Database :
- OpenAIRE
- Journal :
- Radiation Research
- Accession number :
- edsair.doi.dedup.....610da89af19fb964ef0c71d67db0a26f