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EFFECT OF CYANIDE AND ELECTRICAL STIMULATION ON PHOSPHOINOSITIDE METABOLISM IN LOBSTER NERVES
- Source :
- Journal of Neurochemistry. 18:1291-1298
- Publication Year :
- 1971
- Publisher :
- Wiley, 1971.
-
Abstract
- The contents of phosphoinositides, ATP, glucose and lactate in leg and claw nerves of the lobster were determined. Nerves were also analysed after cyanide poisoning, after electrical stimulation, and 1 h after removing the leg from the lobster. Cyanide poisoning decreased the levels of ATP and glucose and increased the content of lactate but did not alter the levels of phosphoinositides. Nerves left in situ for 1 h after disconnection from the central nervous system exhibited a decrease in the content of tri-phosphoinositides (TPI) of 50 per cent, without changes in ATP, glucose or lactate. The TPI change was reversed after incubation for 1 h in oxygenated seawater. Nerves labelled in vivo with 32P were removed and stimulated at 50 Hz for 5 min. The turnover of TPI phosphorus increased on stimulation in both normal and cyanide-poisoned nerves. In contrast, turnover of ATP increased after stimulation in normal nerves but not in cyanide-treated nerves. We sought to determine whether polyphosphoinositides play a greater role in resting metabolism of the nerve or in the conducting mechanisms. Our results make more likely the involvement of TPI in permeability changes of neural membranes during excitation.
- Subjects :
- medicine.medical_specialty
Cell Membrane Permeability
Chromatography, Paper
Cyanide
Central nervous system
Stimulation
In Vitro Techniques
Biology
Phosphatidylinositols
Biochemistry
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Adenosine Triphosphate
In vivo
Crustacea
Internal medicine
medicine
Animals
Peripheral Nerves
Incubation
Phosphoinositide metabolism
Cyanides
Phosphorus Isotopes
Extremities
Metabolism
Electric Stimulation
Glucose
medicine.anatomical_structure
Endocrinology
chemistry
Lactates
Phosphatidylcholines
Cyanide poisoning
Subjects
Details
- ISSN :
- 14714159 and 00223042
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Neurochemistry
- Accession number :
- edsair.doi.dedup.....6135d028f5921c43523264882a87a23c