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Predominant Enhancement of Glucose Uptake in Astrocytes versus Neurons during Activation of the Somatosensory Cortex
- Source :
- Journal of Neuroscience, Journal of Neuroscience, Society for Neuroscience, 2010, 30, pp.15298-15303. ⟨10.1523/jneurosci.0762-10.2010⟩
- Publication Year :
- 2010
- Publisher :
- Society for Neuroscience, 2010.
-
Abstract
- Glucose is the primary energetic substrate of the brain, and measurements of its metabolism are the basis of major functional cerebral imaging methods. Contrary to the general view that neurons are fueled solely by glucose in proportion to their energetic needs, recentin vitroandex vivoanalyses suggest that glucose preferentially feeds astrocytes. However, the cellular fate of glucose in the intact brain has not yet been directly observed. We have used a real-time method for measuring glucose uptake in astrocytes and neuronsin vivoin male rats by imaging the trafficking of the nonmetabolizable glucose analog 6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-aminoglucose (6-NBDG) using two-photon microscopy. During resting conditions we found that astrocytes and neurons both take up 6-NBDG at the same rate in the barrel cortex of the rat. However, during intense neuronal activity triggered by whisker stimulation, astrocytes rapidly accelerated their uptake, whereas neuronal uptake remained almost unchanged. After the stimulation period, astrocytes returned to their preactivation rates of uptake paralleling the neuronal rate of uptake. These observations suggest that glucose is taken up primarily by astrocytes, supporting the view that functional imaging experiments based on glucose analogs extraction may predominantly reflect the metabolic activity of the astrocytic network.
- Subjects :
- Male
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Glucose uptake
Stimulation
Carbohydrate metabolism
Biology
Somatosensory system
Article
Rats, Sprague-Dawley
Animals
Premovement neuronal activity
Neurons
Analysis of Variance
General Neuroscience
Biological Transport
Glucose analog
Somatosensory Cortex
Metabolism
Barrel cortex
Rats
Cell biology
Glucose
Microscopy, Fluorescence, Multiphoton
Astrocytes
Vibrissae
Neuroscience
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....618cbed03de9ca98c32ab4078d7636f9