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Integrative analysis reveals essential mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) in paroxysmal and persistent atrial fibrillation patients
- Source :
- Anatol J Cardiol, Anatolian Journal of Cardiology, Vol 25, Iss 6, Pp 414-428 (2021)
- Publication Year :
- 2021
- Publisher :
- Turkish Society of Cardiology, 2021.
-
Abstract
- OBJECTIVE: This study aimed to investigate the functions of mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) in paroxysmal and persistent atrial fibrillation (AF) patients. METHODS: A total of 9 left atrial appendage (LAA) tissues were collected from patients with AF (ParoAF patients = 3 and PersAF patients = 3) and donors (n=3). Genes and circRNAs were identified by per kilobase per million reads (RPKM) and number of circular reads/number of mapped reads/read length (SRPBM), respectively. Differentially expressed mRNAs (DE mRNAs), lncRNAs (DE lncRNAs), and circRNAs (DE circRNAs) were identified by | log(2) (Fold Change) | ≥ 2 and p-value < 0.05. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Protein-protein, mRNA-lncRNA, and circRNA-miRNA interaction networks were constructed. In addition, logistic analysis was conducted among AF and circRNAs. RESULTS: A total of 285 (116 up-regulated and 169 down-regulated) and 275 (110 up-regulated and 165 down-regulated) DE mRNAs, 575 (276 up-regulated and 299 down-regulated) and 583 (330 up-regulated and 253 down-regulated) DE lncRNAs, and 83 (48 up-regulated and 35 down-regulated) and 99 (58 up-regulated and 41 down-regulated) circRNAs were detected in ParoAF and PersAF, respectively, as compared with control. MAPK signal pathway as well as voltage-dependent, L type, and alpha 1C subunit calcium channel (CACNA1C) might participate in AF occurrence by preventing atrial parasympathetic remodeling. Collagen type I alpha 1 (COL1A1) and COL1A2 mostly participated in the enriched GO and KEGG terms and connected with most of the DE mRNAs. The expression of chr10:69902697|69948883 was a protective factor against PersAF after adjusting for age (p=0.022, 95% CI: 0.003–0.634). CONCLUSION: We found that some mRNAs, lncRNAs, circRNAs, and pathways play essential roles in AF pathogenesis and development. Moreover, one protective factor against PersAF was detected.
- Subjects :
- Messenger RNA
business.industry
RNA
RNA, Circular
Molecular biology
Fold change
Long non-coding RNA
Collagen Type I, alpha 1 Chain
Collagen, type I, alpha 1
MicroRNAs
Circular RNA
RC666-701
Atrial Fibrillation
Diseases of the circulatory (Cardiovascular) system
Medicine
Humans
RNA, Long Noncoding
RNA, Messenger
KEGG
Cardiology and Cardiovascular Medicine
business
Gene
Original Investigation
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Anatol J Cardiol, Anatolian Journal of Cardiology, Vol 25, Iss 6, Pp 414-428 (2021)
- Accession number :
- edsair.doi.dedup.....61ae031fa5635f0b4505bad0bf7864da