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Regulatory functions of l-carnitine, acetyl, and propionyl l-carnitine in a PCOS mouse model: Focus on antioxidant/antiglycative molecular pathways in the ovarian microenvironment
- Source :
- Antioxidants, Volume 9, Issue 9, Antioxidants, Vol 9, Iss 867, p 867 (2020)
- Publication Year :
- 2020
-
Abstract
- Polycystic ovary syndrome (PCOS) is a complex metabolic disorder associated with female infertility. Based on energy and antioxidant regulatory functions of carnitines, we investigated whether acyl-L-carnitines improve PCOS phenotype in a mouse model induced by dehydroepiandrosterone (DHEA). CD1 mice received DHEA for 20 days along with two different carnitine formulations: one containing L-carnitine (LC) and acetyl-L-carnitine (ALC), and the other one containing also propionyl-L-carnitine (PLC). We evaluated estrous cyclicity, testosterone level, ovarian follicle health, ovulation rate and oocyte quality, collagen deposition, lipid droplets, and 17&szlig<br />HSD IV (17 beta-hydroxysteroid dehydrogenase type IV) expression. Moreover, we analyzed protein expression of SIRT1, SIRT3, SOD2 (superoxide dismutase 2), mitochondrial transcriptional factor A (mtTFA), RAGE (receptor for AGEs), GLO2 (glyoxalase 2) and ovarian accumulation of MG-AGEs (advanced glycation end-products formed by methylglyoxal). Both carnitine formulations ameliorated ovarian PCOS phenotype and positively modulated antioxidant molecular pathways in the ovarian microenvironment. Addition of PLC to LC-ALC formulation mitigated intraovarian MG-AGE accumulation and increased mtTFA expression. In conclusion, our study supports the hypothesis that oral administration of acyl-L-carnitines alleviates ovarian dysfunctions associated with this syndrome and that co-administration of PLC provides better activity. Molecular mechanisms underlying these effects include anti-oxidant/glycative activity and potentiation of mitochondria.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
endocrine system
endocrine system diseases
L-carnitine (LC)
Physiology
Propionyl-L-carnitine (PLC)
Clinical Biochemistry
SOD2
Dehydroepiandrosterone
Acetyl-L-carnitine (ALC)
Biochemistry
Article
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
PCOS
acetyl-L-carnitine (ALC)
propionyl-L-carnitine (PLC)
mitochondria
advanced glycation end-products
sirtuins
oocyte quality oxidative stress
glycative stress
Internal medicine
medicine
Sirtuins
Carnitine
Ovarian follicle
Advanced glycation end-products
Molecular Biology
Oocyte quality oxidative stress
030219 obstetrics & reproductive medicine
biology
Glycative stress
Mitochondria
Chemistry
lcsh:RM1-950
Methylglyoxal
Cell Biology
TFAM
Polycystic ovary
lcsh:Therapeutics. Pharmacology
030104 developmental biology
medicine.anatomical_structure
Endocrinology
biology.protein
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Antioxidants, Volume 9, Issue 9, Antioxidants, Vol 9, Iss 867, p 867 (2020)
- Accession number :
- edsair.doi.dedup.....61c3a6a2d42dcfa21888884b4439d3d2