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Testis developmental related gene 1 promotes non-small-cell lung cancer through the microRNA-214-5p/Krüppel-like factor 5 axis
- Source :
- Bioengineered, Vol 13, Iss 1, Pp 603-616 (2022), Bioengineered, article-version (VoR) Version of Record
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- Non-small-cell lung cancer (NSCLC) is a frequent malignancy and has a high global incidence. Long noncoding RNAs (lncRNAs) are implicated in carcinogenesis and tumor progression. LncRNA testis developmental related gene 1 (TDRG1) plays a pivotal role in many cancers. This study researched the biological regulatory mechanisms of TDRG1 in NSCLC. Gene expression was assessed by reverse transcriptase quantitative polymerase chain reaction (RT–qPCR). Changes in the NSCLC cell phenotypes were examined using 5-ethynyl-2ʹ-deoxyuridine (EdU), cell counting kit-8 (CCK-8), wound healing, flow cytometry, and Transwell assays. The binding capacity between TDRG1, microRNA-214-5p (miR‑214-5p), and Krüppel-like factor 5 (KLF5) was tested using luciferase reporter and RNA immunoprecipitation (RIP) assays. In this study, we found that TDRG1 was upregulated in NSCLC samples. Functionally, TDRG1 depletion inhibited NSCLC cell growth, migration, and invasion and accelerated apoptosis. In addition, TDRG1 interacted with miR-214-5p, and miR-214-5p directly targeted KLF5. The suppressive effect of TDRG1 knockdown on NSCLC cellular processes was abolished by KLF5 overexpression. Overall, TDRG1 exerts carcinogenic effects in NSCLC by regulating the miR-214-5p/KLF5 axis.<br />Flow Sheet Abstract
- Subjects :
- Lung Neoplasms
Kruppel-Like Transcription Factors
Bioengineering
Biology
Applied Microbiology and Biotechnology
Krüppel
Carcinoma, Non-Small-Cell Lung
microRNA
medicine
Humans
RNA, Neoplasm
Related gene
Lung cancer
mir-214-5p
General Medicine
medicine.disease
Neoplasm Proteins
respiratory tract diseases
MicroRNAs
A549 Cells
Cancer research
RNA, Long Noncoding
Non small cell
tdrg1
klf5
nsclc
TP248.13-248.65
Signal Transduction
Research Article
Research Paper
Biotechnology
Subjects
Details
- ISSN :
- 21655987 and 21655979
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Bioengineered
- Accession number :
- edsair.doi.dedup.....61c69dedd53ceadfeafad8df8a5d17c6