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Netosis and Inflammasomes in Large Vessel Occlusion Thrombi
- Source :
- Frontiers in Pharmacology, Frontiers in Pharmacology, Vol 11 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- The inflammatory response appears to play a critical role in clotting in which neutrophil extracellular traps (NETs) are the major drivers of thrombosis in acute ischemic stroke (AIS). The inflammasome is an innate immune complex involved in the activation of interleukin (IL)-18 and IL-1β through caspase-1, but whether the inflammasome plays a role in NETosis in AIS remains poorly understood. Here we assessed the levels of inflammasome signaling proteins in NETs and their association with clinical and procedural outcomes of mechanical thrombectomy for AIS. Electron microscopy and immunofluorescence indicate the presence of NETs in thrombi of patients with AIS. Moreover, the inflammasome signaling proteins caspase-1 and apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) were also present in clots associated with the marker of NETosis citrullinated histone 3H (CitH3). Analysis of protein levels by a simple plex assay show that caspase-1, ASC and interleukin (IL)-1β were significantly elevated in clots when compared to plasma of AIS patients and healthy controls, while IL-18 levels were lower. Moreover, multivariate analyses show that IL-1β levels in clots contribute to the number of passes to achieve complete recanalization, and that ASC, caspase-1 and IL-18 are significant contributors to time to recanalization. Thus, inflammasome proteins are elevated in NETs present in thrombi of patients with AIS that contribute to poor outcomes following stroke.
- Subjects :
- Caspase 1
caspase-1
neutrophil extracellular traps
Inflammation
ASC
inflammasome
Medicine
Pharmacology (medical)
Caspase
Original Research
Pharmacology
Innate immune system
biology
business.industry
lcsh:RM1-950
Interleukin
Inflammasome
NETs
Neutrophil extracellular traps
medicine.disease
Thrombosis
stroke
lcsh:Therapeutics. Pharmacology
inflammation
thrombus
Immunology
biology.protein
medicine.symptom
business
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 16639812
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in Pharmacology
- Accession number :
- edsair.doi.dedup.....61fa0a272cbc9af00aa2e45ddba446ad
- Full Text :
- https://doi.org/10.3389/fphar.2020.607287