Back to Search
Start Over
Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences; Volume 18; Issue 2; Pages: 345, International Journal of Molecular Sciences, Vol 18, Iss 2, p 345 (2017)
- Publication Year :
- 2016
-
Abstract
- Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Nanoparticle
Metal Nanoparticles
stem cell tracking
rat subretinal layer
Eye
Catalysis
Article
Immunophenotyping
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
In vivo
transmission electron microscopy
medicine
Cytotoxic T cell
Animals
Physical and Theoretical Chemistry
micro-computed tomography
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
mesenchymal stem cells
Chemistry
Micro computed tomography
Organic Chemistry
Mesenchymal stem cell
General Medicine
X-Ray Microtomography
Computer Science Applications
Rats
gold nanoparticles
030104 developmental biology
Phenotype
lcsh:Biology (General)
lcsh:QD1-999
Colloidal gold
Cell Tracking
Biophysics
Gold
Stem cell
Biomarkers
Homing (hematopoietic)
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 18
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.doi.dedup.....621f616048299720cca9c3ad2e2fbcbd