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Virulence traits of avian pathogenic (APEC) and fecal (AFEC) E. coli isolated from broiler chickens in Algeria
- Source :
- Tropical animal health and production. 50(3)
- Publication Year :
- 2017
-
Abstract
- Avian pathogenic E. coli (APEC) is the etiologic agent of avian colibacillosis, the most common disease responsible for chicken morbidity in the world. Although multiple virulence-associated factors were identified, their prevalence in Algeria is still poorly known. In the present research, 92 avian pathogenic E. coli (APEC) isolates were recovered from broilers with clinical signs and lesions of colibacillosis. In addition, 32 E. coli isolates collected from feces of healthy birds (AFEC) were included for comparison. All isolates were investigated by PCR for the presence of a total of 11 virulence-associated genes described for avian pathogenic (iroN, ompT, hlyF, iss, iutA, and fimC) and diarrheagenic E. coli (eae, stx, elt/est, ipaH, and aggR). The sensitivity of 39 APEC isolates to 16 antibiotics was also determined using antimicrobial pretreated microplates. Here, we report that 98% of the examined isolates host at least one of the tested virulence factors. The most prevalent genes in APEC were iutA (90.6%), ompT (86.9%), and iss (85.8%); whereas, iutA (78.1%), fimC (78.1%), and iroN (68.7%) were the highest prevalent genes in AFEC. Our data showed that none of the AFEC isolates harbor any of the tested diarrheagenic genes. Moreover, only elt/est (5.4%), stx (2.1%), and ipaH (2.1%) genes were carried by APEC isolates. We further established that ceftazodime, ceftiofur, mequindox, amoxicillin/clavulanic acid, and meropenem were the most efficient antibiotics against the analyzed APEC isolates. Overall, our findings provide more insights about APEC and AFEC virulence potential in Algeria which could participate in the fight against colibacillosis.
- Subjects :
- 0301 basic medicine
animal structures
040301 veterinary sciences
medicine.drug_class
Virulence Factors
030106 microbiology
Antibiotics
Virulence
Microbial Sensitivity Tests
Biology
Meropenem
Polymerase Chain Reaction
Microbiology
0403 veterinary science
03 medical and health sciences
Feces
Food Animals
Clavulanic acid
medicine
Escherichia coli
Animals
Escherichia coli Infections
Poultry Diseases
Escherichia coli Proteins
04 agricultural and veterinary sciences
Amoxicillin
Antimicrobial
Anti-Bacterial Agents
Phenotype
Genes, Bacterial
Algeria
Animal Science and Zoology
Ceftiofur
Chickens
medicine.drug
Subjects
Details
- ISSN :
- 15737438
- Volume :
- 50
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Tropical animal health and production
- Accession number :
- edsair.doi.dedup.....622688eff9d76ce99d1d8549a3e735bf