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Genetically-engineered anti-PSMA exosome mimetics targeting advanced prostate cancer in vitro and in vivo
Genetically-engineered anti-PSMA exosome mimetics targeting advanced prostate cancer in vitro and in vivo
- Source :
- Severic, M, Ma, G, Pereira, S G T, Ruiz, A, Cheung, C C L & Al-Jamal, W T 2021, ' Genetically-engineered anti-PSMA exosome mimetics targeting advanced prostate cancer in vitro and in vivo ', Journal of controlled release : official journal of the Controlled Release Society, vol. 330, pp. 101-110 . https://doi.org/10.1016/j.jconrel.2020.12.017
- Publication Year :
- 2020
-
Abstract
- The present work describes the engineering of anti-PSMA peptide-decorated exosome mimetics (EMs) targeting advanced prostate cancer (PC). The targeted EMs were produced from anti-PSMA peptide, WQPDTAHHWATL, expressing U937 monoblastic cells, followed by successive extrusion cycles. The engineered EMs were nanosized, produced at a high yield, and displayed the anti-PSMA peptide, exosomal markers and monocytes proteins on their surface. As anticipated, PSMA-EMs showed increased cellular internalization in PSMA positive PC cell lines (LNCaP and C4-2B), compared to unmodified EMs. Most importantly, higher tumour targeting was observed in solid C4-2B tumours, following intravenous administration, confirming their targeting ability in vivo. Overall, our study indicates that the engineered anti-PSMA peptide-targeted EMs can be a promising drug delivery system for advanced PC.
- Subjects :
- Glutamate Carboxypeptidase II
Male
media_common.quotation_subject
Pharmaceutical Science
Mice, Nude
02 engineering and technology
urologic and male genital diseases
Exosomes
Exosome
03 medical and health sciences
Prostate cancer
Mice
SDG 3 - Good Health and Well-being
In vivo
Cell Line, Tumor
LNCaP
Glutamate carboxypeptidase II
medicine
Animals
Humans
Internalization
030304 developmental biology
media_common
0303 health sciences
Chemistry
Prostatic Neoplasms
Prostate-Specific Antigen
021001 nanoscience & nanotechnology
medicine.disease
In vitro
Drug delivery
Antigens, Surface
Cancer research
0210 nano-technology
Subjects
Details
- ISSN :
- 18734995
- Volume :
- 330
- Database :
- OpenAIRE
- Journal :
- Journal of controlled release : official journal of the Controlled Release Society
- Accession number :
- edsair.doi.dedup.....623bd07eaba346fbbb5154b1c5efca98
- Full Text :
- https://doi.org/10.1016/j.jconrel.2020.12.017