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Genetically-engineered anti-PSMA exosome mimetics targeting advanced prostate cancer in vitro and in vivo

Genetically-engineered anti-PSMA exosome mimetics targeting advanced prostate cancer in vitro and in vivo

Authors :
Maja Severic
Wafa' T Al-Jamal
Amalia Ruiz
Calvin C L Cheung
Guanglong Ma
Sara Pereira
Source :
Severic, M, Ma, G, Pereira, S G T, Ruiz, A, Cheung, C C L & Al-Jamal, W T 2021, ' Genetically-engineered anti-PSMA exosome mimetics targeting advanced prostate cancer in vitro and in vivo ', Journal of controlled release : official journal of the Controlled Release Society, vol. 330, pp. 101-110 . https://doi.org/10.1016/j.jconrel.2020.12.017
Publication Year :
2020

Abstract

The present work describes the engineering of anti-PSMA peptide-decorated exosome mimetics (EMs) targeting advanced prostate cancer (PC). The targeted EMs were produced from anti-PSMA peptide, WQPDTAHHWATL, expressing U937 monoblastic cells, followed by successive extrusion cycles. The engineered EMs were nanosized, produced at a high yield, and displayed the anti-PSMA peptide, exosomal markers and monocytes proteins on their surface. As anticipated, PSMA-EMs showed increased cellular internalization in PSMA positive PC cell lines (LNCaP and C4-2B), compared to unmodified EMs. Most importantly, higher tumour targeting was observed in solid C4-2B tumours, following intravenous administration, confirming their targeting ability in vivo. Overall, our study indicates that the engineered anti-PSMA peptide-targeted EMs can be a promising drug delivery system for advanced PC.

Details

ISSN :
18734995
Volume :
330
Database :
OpenAIRE
Journal :
Journal of controlled release : official journal of the Controlled Release Society
Accession number :
edsair.doi.dedup.....623bd07eaba346fbbb5154b1c5efca98
Full Text :
https://doi.org/10.1016/j.jconrel.2020.12.017