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Different Region Analysis for Genotyping Yersinia pestis Isolates from China
- Source :
- PLoS ONE, PLoS ONE, Vol 3, Iss 5, p e2166 (2008)
- Publication Year :
- 2008
- Publisher :
- Public Library of Science, 2008.
-
Abstract
- Background DFR (different region) analysis has been developed for typing Yesinia pestis in our previous study, and in this study, we extended this method by using 23 DFRs to investigate 909 Chinese Y. pestis strains for validating DFR-based genotyping method and better understanding adaptive microevolution of Y. pestis. Methodology/Principal Findings On the basis of PCR and Bionumerics data analysis, 909 Y. pestis strains were genotyped into 32 genomovars according to their DFR profiles. New terms, Major genomovar and Minor genomovar, were coined for illustrating evolutionary relationship between Y. pestis strains from different plague foci and different hosts. In silico DFR profiling of the completed or draft genomes shed lights on the evolutionary scenario of Y. pestis from Y. pseudotuberculosis. Notably, several sequenced Y. pestis strains share the same DFR profiles with Chinese strains, providing data for revealing the global plague foci expansion. Conclusions/significance Distribution of Y. pestis genomovars is plague focus-specific. Microevolution of biovar Orientalis was deduced according to DFR profiles. DFR analysis turns to be an efficient and inexpensive method to portrait the genome plasticity of Y. pestis based on horizontal gene transfer (HGT). DFR analysis can also be used as a tool in comparative and evolutionary genomic research for other bacteria with similar genome plasticity.
- Subjects :
- Genome evolution
China
Genotype
Yersinia pestis
Biovar
lcsh:Medicine
Pathology/Forensic Pathology
Public Health and Epidemiology/Infectious Diseases
Molecular Biology/Molecular Evolution
Genome
Polymerase Chain Reaction
Typing
lcsh:Science
Genotyping
DNA Primers
Comparative genomics
Genetics
Multidisciplinary
Microbiology/Microbial Evolution and Genomics
biology
Base Sequence
lcsh:R
Genomovar
biology.organism_classification
Genes, Bacterial
Yersinia pseudotuberculosis
lcsh:Q
Genetics and Genomics/Comparative Genomics
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 3
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....62862316144b7ab1b996bd3609e54eda