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MiR-15a Decreases Bovine Mammary Epithelial Cell Viability and Lactation and Regulates Growth Hormone Receptor Expression
- Source :
- Molecules; Volume 17; Issue 10; Pages: 12037-12048, Molecules, Molecules, Vol 17, Iss 10, Pp 12037-12048 (2012)
- Publication Year :
- 2012
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2012.
-
Abstract
- MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate the expression of target genes at the post-transcriptional level by transcript degradation or translational inhibition. The role of bta-miR-15a in bovine mammary gland hasn’t been reported. Using miRNAs prediction software, GHR gene was predicted to be a potential target of bta-miR-15a. In this study, bovine mammary epithelial cell line was used as an in vitro cell model to address the function of bta-miR-15a on bovine mammary epithelial cells. The expression changes of bta-miR-15a and Ghr after bta-miR-15a transfection were detected by qRT-PCR; the expression of GHR protein and casein was detected by western blotting. To determine whether bta-miR-15a can affect cell viability, cells were examined using an electronic Coulter counter (CASY-TT). In conclusion, bta-miR-15a inhibited the expression of casein of bovine mammary epithelial cells, and cell number and viability were reduced by bta-miR-15a expression. Bta-miR-15a inhibited the viability of mammary epithelial cells as well as the expression of GHR mRNA and protein level, therefore suggesting that bta-miR-15a may play an important role in mammary gland physiology.
- Subjects :
- Cell Survival
proliferation
Mammary gland
Pharmaceutical Science
Growth hormone receptor
Biology
Ghr
Article
Analytical Chemistry
lcsh:QD241-441
Mammary Glands, Animal
lcsh:Organic chemistry
Drug Discovery
microRNA
medicine
Animals
Lactation
Viability assay
Physical and Theoretical Chemistry
3' Untranslated Regions
Regulation of gene expression
Messenger RNA
Base Sequence
Three prime untranslated region
bta-miR-15a
Organic Chemistry
Caseins
Epithelial Cells
Receptors, Somatotropin
Transfection
Molecular biology
MicroRNAs
medicine.anatomical_structure
Gene Expression Regulation
Chemistry (miscellaneous)
Molecular Medicine
Cattle
Female
bovine mammary epithelial cell
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules; Volume 17; Issue 10; Pages: 12037-12048
- Accession number :
- edsair.doi.dedup.....628655261b3744fe39659f4586c230f7
- Full Text :
- https://doi.org/10.3390/molecules171012037