Oxytocin/vasopressin-like peptide inotocin regulates cuticular hydrocarbon synthesis and water balancing in ants

Significance Inotocin, the oxytocin/vasopressin-like peptide, is widely conserved in arthropods; however, little is known about its molecular function. Here, we show that, in ants, the expression levels of inotocin and its receptor are correlated with the age of workers and their behavior. We also demonstrate that inotocin signaling is involved in desiccation resistance by regulating the synthesis of cuticular hydrocarbons. We propose that the up-regulation of inotocin and its receptor as workers age and switch tasks from nursing to foraging is a key physiological adaption to survive drier environments outside of the nest.
Oxytocin/vasopressin-like peptides are important regulators of physiology and social behavior in vertebrates. However, the function of inotocin, the homologous peptide in arthropods, remains largely unknown. Here, we show that the level of expression of inotocin and inotocin receptor are correlated with task allocation in the ant Camponotus fellah. Both genes are up-regulated when workers age and switch tasks from nursing to foraging. in situ hybridization revealed that inotocin receptor is specifically expressed in oenocytes, which are specialized cells synthesizing cuticular hydrocarbons which function as desiccation barriers in insects and for social recognition in ants. dsRNA injection targeting inotocin receptor, together with pharmacological treatments using three identified antagonists blocking inotocin signaling, revealed that inotocin signaling regulates the expression of cytochrome P450 4G1 (CYP4G1) and the synthesis of cuticular hydrocarbons, which play an important role in desiccation resistance once workers initiate foraging.