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MicroRNA-34a expression levels in serum and intratumoral tissue can predict bone metastasis in patients with hepatocellular carcinoma
- Source :
- Oncotarget
- Publication Year :
- 2016
- Publisher :
- Impact Journals LLC, 2016.
-
Abstract
- // Zuo-Lin Xiang 1, * , Xiao-Mei Zhao 1, * , Li Zhang 1 , Ping Yang 1 , Jia Fan 2 , Zhao-You Tang 2 , Zhao-Chong Zeng 1 1 Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, China 2 Department of Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China * These authors have contributed equally to this work Correspondence to: Zhao-Chong Zeng, email: zeng.zhaochong@zs-hospital.sh.cn Keywords: hepatocellular carcinoma, bone metastasis, microRNA-34a, serum, tissue microarray Received: July 05, 2016 Accepted: November 07, 2016 Published: November 23, 2016 ABSTRACT Hepatocellular carcinoma (HCC) patients with bone metastasis (BM) suffer from pain and other symptoms that significantly reduce their quality of life. We screened a microRNA (miRNA) microarray to identify potential serum biomarkers for BM in HCC patients. A miRNA microarray was used to screen for BM-related miRNAs in paired serum samples from HCC patients with BM and from HCC patients without BM. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to quantify candidate miRNAs in serum samples from 106 independent HCC patients. Levels of candidate miRNAs in tissue samples from an independent cohort of 296 HCC patients were evaluated by in situ hybridization and intratumoral tissue microarray. The migration and invasion capabilities of HCCLM3 and SMMC-7721 cells were evaluated following treatment with a mimic and an inhibitor of miR-34a. Ninety miRNAs were differentially expressed in sera from HCC patients with BM when compared with sera from non-BM HCC patients ( P < 0.05). Only miR-34a and miR-498 had false discovery rates (FDRs) < 0.05. In cohorts of 106 and 296 HCC patients, we found that reduced serum and intratumoral miR-34a expression levels were independent risk factors for developing BM. Migration and invasion experiments indicated that a reverse correlation existed between miR-34a and HCC tumor migration and invasion. This study demonstrates the potential for the use of miR-34a as a serum and intratumoral tissue biomarker for predicting the risk of BM in HCC patients.
- Subjects :
- 0301 basic medicine
Oncology
Pathology
medicine.medical_specialty
Microarray
03 medical and health sciences
0302 clinical medicine
Internal medicine
microRNA-34a
medicine
bone metastasis
Tissue microarray
tissue microarray
business.industry
Bone metastasis
hepatocellular carcinoma
medicine.disease
digestive system diseases
030104 developmental biology
Real-time polymerase chain reaction
MicroRNA 34a
030220 oncology & carcinogenesis
Hepatocellular carcinoma
Biomarker (medicine)
Liver cancer
business
serum
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 19492553
- Volume :
- 7
- Issue :
- 52
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....62920e5048f989d1f0a115b5970f36a8