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Genome-lamina interactions are established de novo in the early mouse embryo
- Source :
- Nature, 569(7758), 729-733. Nature Publishing Group, Nature, Nature 569, 729-733 (2019)
- Publication Year :
- 2019
-
Abstract
- In mammals, the emergence of totipotency after fertilization involves extensive rearrangements of the spatial positioning of the genome1,2. However, the contribution of spatial genome organization to the regulation of developmental programs is unclear3. Here, we have generated high-resolution maps in mouse pre-implantation embryos of genomic interactions with the nuclear lamina, a filamentous meshwork that lines the inner-nuclear membrane. We find that nuclear organisation is not inherited from the maternal germline but is instead established de novo rapidly after fertilisation. The two parental genomes establish lamina-associated domains (LADs4) with different features that converge after the 8-cell stage. We find that the mechanism of LAD establishment is unrelated to DNA replication. Instead, we show that paternal LAD formation in zygotes is prevented by ectopic expression of Kdm5b, suggesting that LAD establishment may be dependent upon remodelling of H3K4 methylation. Together, our data suggest a step-wise assembly model whereby early LAD formation precedes consolidation of Topologically Associating Domains (TADs).
- Subjects :
- Male
Jumonji Domain-Containing Histone Demethylases
Zygote
Embryonic Development
Mammalian embryology
Biology
Genome
Article
Germline
Mice
03 medical and health sciences
0302 clinical medicine
Animals
Inner membrane
Chromosome Positioning
030304 developmental biology
Genomic organization
0303 health sciences
Nuclear Lamina
Multidisciplinary
Embryo, Mammalian
Cell biology
DNA-Binding Proteins
Mice, Inbred C57BL
Fertilization
Oocytes
Nuclear lamina
Female
Ectopic expression
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 00280836
- Database :
- OpenAIRE
- Journal :
- Nature, 569(7758), 729-733. Nature Publishing Group, Nature, Nature 569, 729-733 (2019)
- Accession number :
- edsair.doi.dedup.....629e3b13bed3682eebe9e0e9de949033