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A-24, a steroidal saponin from Allium chinense, induced apoptosis, autophagy and migration inhibition in p53 wild-type and p53-deficient gastric cancer cells
- Source :
- Chemico-biological interactions. 348
- Publication Year :
- 2021
-
Abstract
- Allium chinense is a vegetable with nutrition and unique flavor, and it is used as traditional Chinese medicine. We previously reported that the active compound A-24 induces apoptosis and autophagy in p53 wild-type gastric cancer cells through the PI3K/Akt/mTOR pathway. Our present work indicates that A-24 also has a significant proliferation inhibition effect on p53-deficient KATO-III cells, and the p53 status did not affect A-24 induced migration inhibition, but negatively controlled the occurrence of autophagy. We also found that the accumulation of reactive oxygen species (ROS) mediated A-24 induced apoptosis is p53-independent. Besides, p-Akt was not downregulated by A-24 in p53-deficient gastric cancer cells. Taken together, our results indicate that A-24 induced apoptosis and autophagy via the ROS-PI3K/Akt/mTOR pathway in p53 wild-type gastric cancer cells and through the ROS-mTOR pathway in p53-deficient gastric cancer cells. Our study recommended A-24 as a promising future phytotherapeutic candidate for gastric cancer treatment.
- Subjects :
- Saponin
Apoptosis
Toxicology
Allium
Cell Movement
Stomach Neoplasms
Cell Line, Tumor
Autophagy
Humans
Protein kinase B
PI3K/AKT/mTOR pathway
Cell Proliferation
chemistry.chemical_classification
Reactive oxygen species
Chemistry
Wild type
General Medicine
Saponins
Cancer cell
Cancer research
Tumor Suppressor Protein p53
Reactive Oxygen Species
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 18727786
- Volume :
- 348
- Database :
- OpenAIRE
- Journal :
- Chemico-biological interactions
- Accession number :
- edsair.doi.dedup.....62a941bc137d5c397c36b51b39e818d5