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Breed and Feeding System Impact the Bioactive Anti-Inflammatory Properties of Bovine Milk

Authors :
Angela Salzano
Maria Chiara Di Meo
Nunzia D’Onofrio
Giovanna Bifulco
Alessio Cotticelli
Francesca Licitra
Antonio Iraci Fuintino
Giuseppe Cascone
Maria Luisa Balestrieri
Ettore Varricchio
Giuseppe Campanile
Salzano, A
Di Meo, Mc
D'Onofrio, N
Bifulco, G
Cotticelli, A
Licitra, F
Iraci Fuintino, A
Cascone, G
Balestrieri, Ml
Varricchio, E
Campanile, G.
Salzano, Angela
Chiara Di Meo, Maria
D'Onofrio, Nunzia
Bifulco, Giovanna
Cotticelli, Alessio
Licitra, Francesca
Iraci Fuintino, Antonio
Cascone, Giuseppe
Balestrieri, MARIA LUISA
Varricchio, Ettore
Campanile, Giuseppe
Source :
International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11088
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

In the present study, we aimed at assessing the influence of breed and feeding system on the bovine milk profile of betaines and carnitines and milk capacity in counteracting the inflammatory endothelial cell (EC) damage induced by interleukin (IL)-6. In the first experimental design, two breeds were chosen (Holstein vs. Modicana) to investigate the biomolecule content and antioxidant capacity in milk and dairy products. In the second experimental design, two feeding systems (pasture vs. total mixed ratio) were tested only in Holstein to evaluate the possible effect on the functional profile of milk and dairy products. Finally, the bulk milk from the two experimental designs was used to evaluate the efficacy of preventing IL-6-induced endothelial inflammatory damage. Results showed that Modicana milk and whey had higher biomolecule content and antioxidant activity compared to Holstein milk (p < 0.01). Milk from Holstein fed TMR showed higher concentration of γ-butyrobetaine, δ-valerobetaine (p < 0.01), and l-carnitine (p < 0.05). Similarly, whey from Holstein fed TMR also showed higher content of δ-valerobetaine, glycine betaine, l-carnitine, and acetyl-l-carnitine (p < 0.01) compared to the Holstein fed pasture. Conversely, the antioxidant activity of milk and dairy products was not affected by the feeding system. In ECs, all milk samples reduced the IL-6-induced cytokine release, as well as the accumulation of reactive oxygen species (ROS) and the induction of cell death, with the most robust effect elicited by Modicana milk (p < 0.01). Overall, Modicana milk showed a higher content of biomolecules and antioxidant activity compared to Holstein, suggesting that the breed, more than the feeding system, can positively affect the health-promoting profile of dairy cattle milk.

Details

ISSN :
14220067
Volume :
23
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences
Accession number :
edsair.doi.dedup.....62ca4eca984fdb1c5710df3690bdf533
Full Text :
https://doi.org/10.3390/ijms231911088