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Evaluation of the BinaxNOW PBP2a assay for the direct detection of methicillin resistance in Staphylococcus aureus from positive blood culture bottles
Evaluation of the BinaxNOW PBP2a assay for the direct detection of methicillin resistance in Staphylococcus aureus from positive blood culture bottles
- Source :
- Diagnostic Microbiology and Infectious Disease. 72:282-284
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- BinaxNOW® PBP2a rapid immunochromatographic assay is a novel test for the identification of methicillin resistance in Staphylococcus aureus from clinical blood culture samples based on detection of penicillin binding protein 2a. We have evaluated the utility of this assay to do a rapid diagnostic of methicillin susceptibility directly from blood culture bottles after identification of S. aureus in positive bottles by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry. Twenty of 21 methicillin-resistant S. aureus (MRSA) samples from blood cultures were positive on direct immunochromatographic testing (sensitivity 95.24%, 95% confidence interval [CI] 74.13% to 99.75%), whereas 37 methicillin-susceptible S. aureus (MSSA) samples were negative (specificity 100%, 95% CI 88.99% to 99.75%). The combined use of MALDI-TOF mass spectrometry and BinaxNOW® PBP2a test is useful for the rapid identification of both MRSA and MSSA from blood cultures.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
Microbiology (medical)
Penicillin binding proteins
Combined use
medicine.disease_cause
Methicillin resistance
Chromatography, Affinity
Microbiology
Humans
Penicillin-Binding Proteins
Medicine
Blood culture
medicine.diagnostic_test
business.industry
General Medicine
Staphylococcal Infections
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Molecular Typing
Rapid identification
Infectious Diseases
Blood culture bottles
Staphylococcus aureus
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Positive blood culture
Reagent Kits, Diagnostic
business
Subjects
Details
- ISSN :
- 07328893
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Diagnostic Microbiology and Infectious Disease
- Accession number :
- edsair.doi.dedup.....62dc31bb2a91f3ce6825eebe42879146
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2011.11.012