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Evaluation of the BinaxNOW PBP2a assay for the direct detection of methicillin resistance in Staphylococcus aureus from positive blood culture bottles

Evaluation of the BinaxNOW PBP2a assay for the direct detection of methicillin resistance in Staphylococcus aureus from positive blood culture bottles

Authors :
Carolina Navarro
María Pilar Romero-Gómez
Rosa Gómez-Gil
José Ramón Paño-Pardo
Jesús Mingorance
Inmaculada Quiles-Melero
Source :
Diagnostic Microbiology and Infectious Disease. 72:282-284
Publication Year :
2012
Publisher :
Elsevier BV, 2012.

Abstract

BinaxNOW® PBP2a rapid immunochromatographic assay is a novel test for the identification of methicillin resistance in Staphylococcus aureus from clinical blood culture samples based on detection of penicillin binding protein 2a. We have evaluated the utility of this assay to do a rapid diagnostic of methicillin susceptibility directly from blood culture bottles after identification of S. aureus in positive bottles by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry. Twenty of 21 methicillin-resistant S. aureus (MRSA) samples from blood cultures were positive on direct immunochromatographic testing (sensitivity 95.24%, 95% confidence interval [CI] 74.13% to 99.75%), whereas 37 methicillin-susceptible S. aureus (MSSA) samples were negative (specificity 100%, 95% CI 88.99% to 99.75%). The combined use of MALDI-TOF mass spectrometry and BinaxNOW® PBP2a test is useful for the rapid identification of both MRSA and MSSA from blood cultures.

Details

ISSN :
07328893
Volume :
72
Database :
OpenAIRE
Journal :
Diagnostic Microbiology and Infectious Disease
Accession number :
edsair.doi.dedup.....62dc31bb2a91f3ce6825eebe42879146
Full Text :
https://doi.org/10.1016/j.diagmicrobio.2011.11.012