Back to Search
Start Over
Flow cytometric prescreening of cervical smears
- Source :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 27(1)
- Publication Year :
- 1979
-
Abstract
- One-parameter (nuclear DNA) and two-parameter (nuclear DNA and protein or cellular light scatter) measurements of cervical smears were performed using an ICP 11 and a cytofluorograf 4800 respectively. A total of about 1000 cases was analyzed. For the estimation of nuclear DNA alone two fluorochromes were tested (ethidium bromide (EB) and mithramycin (MMC)) combined with three different methods of cell preparation. For the two-parameter measurements cells were double stained with EB and fluorescein isothiocyanate (FITC). Red fluorescence (EB) versus green fluorescence (FITC) or red fluorescence versus scatter were recorded. A computer analysis of the one-parameter histograms was performed using discriminant analysis and the results were compared with the cytodiagnosis of microscopic specimens stained with the Papanicolaou technique. The error rates of the flow cytometric (FCM) data were as follows: (a) standard EB staining, 11% false negative, 26% false positive, 6% unsatisfactory results; (b) pepsination of vital cells and EB staining, 12% false negative, 14% false positive and 4% unsatisfactory results; (c) MMC staining, 10% false negative, 65% false positive and 5% unsatisfactory results. Our two-parameter measurements prove that, as confirmed by cell sorting, red fluorescence versus scatter allows separation of at least three subpopulations in most analyzed samples: (a) anucleated cells; (b) leukocytes; and (c) intermediate and superficial cells.
- Subjects :
- Pathology
medicine.medical_specialty
Histology
Cytological Techniques
Papanicolaou stain
Uterine Cervical Neoplasms
Cervix Uteri
Diagnosis, Differential
Photometry
chemistry.chemical_compound
medicine
Humans
Fluorescein isothiocyanate
Cell Nucleus
Vaginal Smears
Staining and Labeling
Proteins
Papanicolaou Test
DNA
Cell sorting
Fluorescence
Nuclear DNA
Staining
chemistry
Female
Anatomy
Ethidium bromide
Subjects
Details
- ISSN :
- 00221554
- Volume :
- 27
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- Accession number :
- edsair.doi.dedup.....6302961960fcee581d3f12163599cb22