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New genetic lineage within the Siberian subtype of tick-borne encephalitis virus found in Western Siberia, Russia

Authors :
Artem Tikunov
Galina S. Chicherina
Victor V. Glupov
Polina S. Belokopytova
Sergey E. Tkachev
Oksana V. Zadora
Irina Golovljova
Nina V. Tikunova
Source :
Infection, Genetics and Evolution. 56:36-43
Publication Year :
2017
Publisher :
Elsevier BV, 2017.

Abstract

Tick-borne encephalitis virus (TBEV), a member of the Flaviviridae family, is a causative agent of a severe neurological disease. There are three main TBEV subtypes: the European (TBEV-Eu), Far Eastern (TBEV-FE), and Siberian (TBEV-Sib). Currently, three lineages within TBEV-Sib have been recorded. In this study, the genetic and biological characteristics of a new original strain, TBEV-2871, isolated in the Novosibirsk province of Western Siberia, Russia were investigated. The strain has low neuroinvasiveness in mice. Phylogenetic analysis demonstrated that TBEV-2871 belongs to TBEV-Sib, but does not cluster with any of the TBEV-Sib lineages. The TBEV-2871 strain has 88-89% nucleotide sequence identity with the other TBEV-Sib strains, 84-86% nucleotide sequence identity with the TBEV-FE and TBEV-Eu subtypes and is genetically close to the subtype division border. The TBEV-2871 polyprotein sequence includes 43 unique amino acid substitutions, 30 of which are recorded at positions that are conserved among all TBEV subtypes. Strain TBEV-2871 and two similar but not identical isolates found in Kemerovo province, Western Siberia are separated into a new lineage tentatively named Obskaya after the name of Ob riber, in the vicinity of which the TBEV-2871 was first found. A molecular evolution investigation demonstrated that within TBEV-Sib, the Obskaya lineage likely separated 1535years ago, which is even earlier than the Baltic lineage.

Details

ISSN :
15671348
Volume :
56
Database :
OpenAIRE
Journal :
Infection, Genetics and Evolution
Accession number :
edsair.doi.dedup.....630a9293c30b1815aafb58187d762b4a
Full Text :
https://doi.org/10.1016/j.meegid.2017.10.020