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The Cellular and Molecular Mechanisms Underlying Silver Nanoparticle/Chitosan Oligosaccharide/Poly(vinyl alcohol) Nanofiber-Mediated Wound Healing
- Source :
- Journal of Biomedical Nanotechnology. 13:17-34
- Publication Year :
- 2017
- Publisher :
- American Scientific Publishers, 2017.
-
Abstract
- Wound healing is a complex pathophysiological process that occurs frequently in everyday pathology and remains a challenge during the treatment of trauma. Previously, we prepared silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) (PVA/COS-AgNP) nanofibers via an electrospinning technique. These nanofibers promoted the proliferation of human skin fibroblasts (HSFs) and the expression of transforming growth factor TGF-β1 in the early stage of wound repair, although the specific mechanisms remain unclear. Therefore, considering that TGF-β1 has emerged as a major modulator of wound healing, the objective of this study was to further understand whether the molecular mechanisms responsible for PVA/COS-AgNP nanofiber-mediated wound healing include the TGF-β1/Smad signal transduction pathway. In this study, we used human skin fibroblasts (HSFs) to investigate the molecular and cellular mechanisms underlying PVA/COSAgNP nanofiber-mediated wound healing. Cell adhesion and proliferation experiments, immunofluorescence staining, hydroxyproline content measurements, flow cytometry, quantitative real-time PCR (qRT-PCR), and western blotting (WB) were used to analyze the wound healing mechanisms of human skin fibroblasts treated with various concentrations of PVA/COS-AgNP nanofibers and the combined application of silver nanofibers and SB431542 (an inhibitor of the TGF-β1 receptor kinase). Our study showed that PVA/COS-AgNP nanofibers markedly promoted fibroblast proliferation, collagen synthesis, and cell adherence. We also found that treating fibroblasts with PVA/COS-AgNP nanofibers stimulated cell cycle progression from G1 into the S and G2 phases, reducing the proportion of cells in the G0/G1 phase and inducing S and G2/M arrest. Importantly, the cell factors associated with the TGF-β1/Smad signal transduction pathway, such as TGF-β1, TGFβRI, TGFβRII, pSmad2, pSmad3, collagen I, collagen III, and fibronectin were also up-regulated. Moreover, this enhancing effect was markedly inhibited by the TGFβRI receptor inhibitor, SB431542. Therefore, the PVA/COS-AgNP nanofibers used to accelerate wound healing do so by activating the TGF-β1/Smad signal transduction pathway.
- Subjects :
- Silver
Materials science
Nanofibers
Biomedical Engineering
Metal Nanoparticles
Pharmaceutical Science
Medicine (miscellaneous)
Smad Proteins
Bioengineering
02 engineering and technology
SMAD
010402 general chemistry
01 natural sciences
Cell Line
Transforming Growth Factor beta1
Polymer chemistry
Cell Adhesion
medicine
Humans
General Materials Science
Cell adhesion
Fibroblast
Cell Proliferation
Chitosan
Wound Healing
integumentary system
biology
Electrochemical Techniques
Fibroblasts
021001 nanoscience & nanotechnology
0104 chemical sciences
Cell biology
Fibronectin
medicine.anatomical_structure
Polyvinyl Alcohol
Nanofiber
biology.protein
Signal transduction
0210 nano-technology
Wound healing
Signal Transduction
Transforming growth factor
Subjects
Details
- ISSN :
- 15507033
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Nanotechnology
- Accession number :
- edsair.doi.dedup.....630be3b888e72f08bb6ecb1a9e62ba57
- Full Text :
- https://doi.org/10.1166/jbn.2017.2324