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Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis
- Source :
- PLoS ONE, Vol 16, Iss 6, p e0253502 (2021), PLoS ONE
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Saliva contains diverse bacteria shed from various oral sites, including subgingival plaque. It is reasonable to focus on the total occupancy of subgingival plaque-specific bacteria (SUBP bacteria), which live in subgingival environments, in the saliva for detecting periodontitis using salivary testing. This study aimed to validate the clinical utility of SUBP bacteria in the salivary microbiota for the detection of periodontitis. We examined stimulated saliva samples collected from 125 subjects who visited three dental clinics. The relative abundances of previously identified 11 SUBP bacteria were determined using 16S ribosomal RNA gene sequencing and a reference-based approach. The prediction performance was evaluated using a receiver operating characteristic (ROC) curve. The SUBP bacteria accounted for 0–15.4% of the salivary microbiota, and the percentage distinguished periodontitis patients with at least 15 sites with probing depth ≥4 mm with a sensitivity of 0.90 (95% confidence interval [CI], 0.81–0.98) and specificity of 0.70 (95% CI, 0.60–0.80) (area under the ROC curve [AUC], 0.87). Among 2,047 combinations of 11 SUBP bacteria, combinations including Streptococcus constellatus, Porphyromonas gingivalis, and Fusobacterium nucleatum subsp. vincentii demonstrated significantly higher AUC values in their detection. These results suggest that examining SUBP bacteria in saliva may be useful for detecting periodontitis patients in mass screening.
- Subjects :
- Male
Saliva
Physiology
Pathology and Laboratory Medicine
Biochemistry
Medical Conditions
Oral Diseases
RNA, Ribosomal, 16S
Medicine and Health Sciences
Mass Screening
Multidisciplinary
biology
Ecology
Microbiota
Genomics
Middle Aged
Streptococcus constellatus
Body Fluids
Bacterial Pathogens
Nucleic acids
RNA, Bacterial
Ribosomal RNA
Medical Microbiology
Medicine
Female
Anatomy
Pathogens
Research Article
Adult
Cell biology
Cellular structures and organelles
Science
Oral Medicine
Microbial Genomics
Microbiology
Ecosystems
Fusobacteria
Microbial Ecosystems
medicine
Genetics
Humans
Periodontitis
Non-coding RNA
Porphyromonas gingivalis
Microbial Pathogens
Mass screening
Periodontal Diseases
Aged
Bacteria
business.industry
Ecology and Environmental Sciences
Gut Bacteria
Organisms
Biology and Life Sciences
medicine.disease
biology.organism_classification
RNA
Microbiome
Fusobacterium nucleatum
business
Ribosomes
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....63164d21645c340e202edac8ff27cedd