Back to Search
Start Over
miR‐212‐5p exerts tumor promoter function by regulating the Id3/PI3K/Akt axis in lung adenocarcinoma cells
- Source :
- Journal of Cellular Physiology. 235:7273-7282
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- microRNAs may function as oncogenes or tumor suppressor genes that play crucial roles in human carcinogenesis and cancer development. Growing evidence revealed that the tumor suppressor Id3 is involved in tumor progression, carcinogenesis, and the tumor microenvironment. We identified miR-212-5p as a negative posttranscriptional modulator of Id3. Dual luciferase reporter assay was used to verify that Id3 is a direct target gene of miR-212-5p. Id3 was lowly expressed and miR-212-5p was highly expressed in non-small-cell lung cancer (NSCLC) tissues and cells. In addition, we found that NSCLC patients having a higher level of miR-212-5p expression had a shorter survival time. Besides this, miR-212-5p could directly target Id3 and reduce its expression. miR-212-5p overexpression significantly accelerated cell proliferation, migration, and invasion by reversing the effects of Id3. Id3 overexpression by silencing miR-212-5p expression suppressed phosphatidylinositol 3 kinase (PI3K)/Akt activity and consequently promoted apoptosis and inhibited cell proliferation in lung cancer cells. Consistent with the in vitro results, a xenograft mouse model was used to validate the fact that miR-212-5p could promote tumorigenesis by targeting Id3 and activate the PI3K/Akt pathway in vivo as well. Taken together, the present results indicated that miR-212-5p may be involved in progression of NSCLC through the PI3K/Akt signaling pathway by targeting Id3.
- Subjects :
- Male
0301 basic medicine
Lung Neoplasms
Carcinogenesis
Physiology
Clinical Biochemistry
Adenocarcinoma of Lung
Apoptosis
Biology
medicine.disease_cause
Mice
Phosphatidylinositol 3-Kinases
03 medical and health sciences
0302 clinical medicine
Cell Movement
Carcinoma, Non-Small-Cell Lung
microRNA
medicine
Animals
Humans
Gene silencing
Neoplasm Invasiveness
Protein kinase B
PI3K/AKT/mTOR pathway
Cell Proliferation
Mice, Inbred BALB C
Tumor microenvironment
Akt/PKB signaling pathway
Cell Biology
Prognosis
Neoplasm Proteins
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
Tumor progression
030220 oncology & carcinogenesis
Carcinogens
Cancer research
Heterografts
Inhibitor of Differentiation Proteins
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 235
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....631eb905400d3b4a7ea099590b242773