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Intracerebral complement C5a receptor (CD88) expression is regulated by TNF and lymphotoxin-α following closed head injury in mice
- Source :
- Journal of Neuroimmunology. 109:164-172
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- The anaphylatoxin C5a is a potent mediator of inflammation in the CNS. We analyzed the intracerebral expression of the C5a receptor (C5aR) in a model of closed head injury (CHI) in mice. Up-regulation of C5aR mRNA and protein expression was observed mainly on neurons in sham-operated and head-injured wild-type mice at 24 h. In contrast, in TNF/lymphotoxin-alpha knockout mice, the intracerebral C5aR expression remained at low constitutive levels after sham operation, whereas it strongly increased in response to trauma between 24 and 72 h. Interestingly, by 7 days after CHI, the intrathecal C5aR expression was clearly attenuated in the knockout animals. These data show that the posttraumatic neuronal expression of the C5aR is, at least in part, regulated by TNF and lymphotoxin-alpha at 7 days after trauma.
- Subjects :
- Lymphotoxin alpha
medicine.medical_specialty
Immunology
Gene Expression
Inflammation
Biology
C5a receptor
Mice
Antigens, CD
Head Injuries, Closed
Internal medicine
Gene expression
medicine
Animals
Immunology and Allergy
RNA, Messenger
Receptor
Lymphotoxin-alpha
Receptor, Anaphylatoxin C5a
In Situ Hybridization
Brain Chemistry
Mice, Knockout
Tumor Necrosis Factor-alpha
Receptors, Complement
Mice, Inbred C57BL
Endocrinology
Lymphotoxin
Neurology
Knockout mouse
Tumor necrosis factor alpha
Neurology (clinical)
medicine.symptom
Subjects
Details
- ISSN :
- 01655728
- Volume :
- 109
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroimmunology
- Accession number :
- edsair.doi.dedup.....6360200dddc21d32099052fca69e8f50