Back to Search
Start Over
The two major subspecies of Fusobacterium necrophorum have distinct leukotoxin operon promoter regions
- Source :
- Veterinary Microbiology. 112:73-78
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- Fusobacterium necrophorum, a gram-negative, non-spore-forming anaerobe, is a normal inhabitant of the alimentary tract of animals and humans. Two types of F. necrophorum, subspecies necrophorum (biotype A) and funduliforme (biotype B), have been recognized, which differ morphologically, biochemically and biologically. The organism is an opportunistic pathogen that causes numerous necrotic conditions (necrobacillosis) such as bovine hepatic abscesses and ruminant foot abscesses. Subspecies necrophorum strains are considered to be more virulent for cattle and have been shown to produce greater amounts of leukotoxin than subspecies funduliforme strains. The leukotoxin operon of F. necrophorum consists of three genes (lktBAC) of which the leukotoxin structural gene (lktA) is the second gene in the operon. In this study, the promoter regions of the leukotoxin operons from the two subspecies were identified and their nucleotide sequence compared. The promoter regions were found to differ in sequence, in length of the sequence between the upstream determinant (oppF) and the first gene of the leukotoxin operon (lktB), and in promoter strength as assayed in Escherichia coli host cells.
- Subjects :
- DNA, Bacterial
Sequence analysis
Operon
Bacterial Toxins
Molecular Sequence Data
ved/biology.organism_classification_rank.species
Cattle Diseases
Exotoxins
Virulence
Biology
medicine.disease_cause
Microbiology
Species Specificity
Sequence Homology, Nucleic Acid
Fusobacterium necrophorum
medicine
Animals
Cloning, Molecular
Promoter Regions, Genetic
Gene
Escherichia coli
Phylogeny
Genetics
Base Sequence
General Veterinary
ved/biology
Structural gene
Gene Amplification
Nucleic acid sequence
Sequence Analysis, DNA
General Medicine
Fusobacterium Infections
Cattle
Subjects
Details
- ISSN :
- 03781135
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Veterinary Microbiology
- Accession number :
- edsair.doi.dedup.....636ec83cd51e355548251b0e56348d93