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Expression and clinicopathological significance of lipinā1 in human breast cancer and its association with p53 tumor suppressor gene
- Source :
- Journal of Cellular Physiology. 235:5835-5846
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Breast cancer (BC) is an important cause of female cancer-related death. It has recently been demonstrated that metabolic disorders including lipid metabolism are a hallmark of cancer cells. Lipin-1 is an enzyme that displays phosphatidate phosphatase activity and regulates the rate-limiting step in the pathway of triglycerides and phospholipids synthesis. The objective of this study was to evaluate lipin-1 expression, its prognostic significance, and its correlation with p53 tumor suppressor in patients with BC. In this study, 55 pairs of fresh samples of BC and adjacent noncancerous tissue were used to analyze lipin-1, using quantitative real-time polymerase chain reaction and immunohistochemistry (IHC) staining. The expression of other clinicopathological variables and p53 was also examined using IHC technique. The cell migration was studied in MCF-7 and MDA-MB231 cells following the inhibition of lipin-1 by propranolol. Our results show that the relative expression of lipin-1 messenger RNA was significantly higher in BC tissues compared with the adjacent normal tissue and its inhibition reduced cell migration in cancer cells. This upregulation was negatively correlated with histological grade of tumor and p53 status (p = .001 and p = .034) respectively and positively correlated with the tumor size (p = .006). Our results also seem to indicate that the high lipin-1 expression is related to a good prognosis in patients with BC. The expression of lipin-1 may be considered as a novel independent prognostic factor. The inhibition of lipin-1 may also have therapeutic significance for patients with BC. The correlation between lipin-1 and p53 confirms the role of p53 in the regulation of lipid metabolism in cancer cells.
- Subjects :
- 0301 basic medicine
Physiology
Clinical Biochemistry
Phosphatidate Phosphatase
Breast Neoplasms
Biology
Disease-Free Survival
03 medical and health sciences
0302 clinical medicine
Breast cancer
Downregulation and upregulation
Cell Movement
medicine
Humans
Triglycerides
Cell Proliferation
Messenger RNA
Lipogenesis
Cancer
Lipid metabolism
Cell migration
Cell Biology
Middle Aged
Lipid Metabolism
Prognosis
medicine.disease
Gene Expression Regulation, Neoplastic
030104 developmental biology
030220 oncology & carcinogenesis
Cancer cell
Cancer research
Immunohistochemistry
Female
Tumor Suppressor Protein p53
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 235
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....63bf204e6cf7bd263cc37951c9a9901a