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Electrospun Heparin-Loaded Core-Shell Nanofiber Sutures for Achilles Tendon Regeneration In Vivo
Electrospun Heparin-Loaded Core-Shell Nanofiber Sutures for Achilles Tendon Regeneration In Vivo
- Source :
- Macromolecular bioscience. 18(7)
- Publication Year :
- 2018
-
Abstract
- Achilles tendon reconstruction surgery is the primary clinical method for repairing acute Achilles tendon ruptures. However, the efficacy of the postoperative healing process and the recovery of physiological function are inadequate. This study examines the healing mechanism of ruptured rat Achilles tendons seamed with heparin-loaded core-shell fiber sutures fabricated via near-field electrospinning. High-heparin-concentration sutures (PPH3.0) perform better than the low-heparin-concentration sutures and commercial sutures (CSs). The PPH3.0 suture recruits fewer inflammatory cells and shows good histocompatibility in peritoneal implantation experiments. Staining of the Achilles tendon rupture repair zone demonstrates that a high heparin concentration in sutures reduces immune-inflammatory responses. Immunohistochemical analysis reveals that the transforming growth factor-β staining scores of the PPH3.0 sutures are not significantly different from those of the corresponding control group but are significantly different from those of the CSs and non-heparin-loaded-suture groups. According to vascular endothelial growth factor (VEGF) analysis, the concentration of VEGF in the group treated with the PPH3.0 suture increases by 37.5% compared with that in its control group. No significant difference in tension strength is observed between the PPH3.0 group and healthy Achilles tendons. These findings illustrate that this novel method effectively treats Achilles tendon rupture and promotes healing and regeneration.
- Subjects :
- 0301 basic medicine
Male
Vascular Endothelial Growth Factor A
medicine.medical_specialty
Polymers and Plastics
Nanofibers
Gene Expression
Bioengineering
02 engineering and technology
Achilles Tendon
Biomaterials
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Suture (anatomy)
In vivo
Tendon Injuries
Transforming Growth Factor beta
Tensile Strength
Materials Chemistry
Medicine
Animals
Humans
Rupture
Achilles tendon
Wound Healing
Sutures
business.industry
Guided Tissue Regeneration
Heparin
Regeneration (biology)
Suture Techniques
Electrochemical Techniques
021001 nanoscience & nanotechnology
Surgery
Rats
Vascular endothelial growth factor
030104 developmental biology
medicine.anatomical_structure
chemistry
Nanofiber
Achilles tendon rupture
medicine.symptom
0210 nano-technology
business
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 16165195
- Volume :
- 18
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Macromolecular bioscience
- Accession number :
- edsair.doi.dedup.....63c3b5ecd2d3a1062f3293f445574057