Back to Search
Start Over
Determination of antimicrobial MIC by paper diffusion method
- Source :
- Journal of clinical pathology. 28(12)
- Publication Year :
- 1975
-
Abstract
- Because they are cumbersome, tests to determine the quantitative susceptibility of organisms to antimicrobial drugs are not performed routinely in many diagnostic laboratories. This paper describes a simple method of incorporating the antimicrobial drug in agar. It is an adaptation of the Rolinson and Russell technique which allows the determination of minimum inhibitory concentrations (MIC) of antimicrobial drugs for a large number of organisms. Results are comparable with those obtained when the standard agar dilution method is used. Strains of aerobic Gram-negative bacilli were tested by both methods using ampicillin (86 strains), cephaloridine (72 strains), trimethoprim (72 strains), and gentamicin (72 strains). Of the 302 tests thus performed, a difference in MIC of more than one double dilution was noted in only 11 tests. With one strain of Pseudomonas aeruginosa, however, it was not possible to detect ampicillin resistance by the method described in this paper.
- Subjects :
- Paper
food.ingredient
medicine.drug_class
Cephalosporin
Antibiotics
Microbial Sensitivity Tests
Biology
Trimethoprim
Pathology and Forensic Medicine
Microbiology
Diffusion
Minimum inhibitory concentration
food
Amp resistance
Ampicillin
Klebsiella
medicine
Escherichia coli
Agar
General Medicine
Antimicrobial
Anti-Bacterial Agents
Pseudomonas aeruginosa
Cephaloridine
Gentamicin
Gentamicins
medicine.drug
Research Article
Subjects
Details
- ISSN :
- 00219746
- Volume :
- 28
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of clinical pathology
- Accession number :
- edsair.doi.dedup.....63fc20ae8f64962d7886fbff5b793949