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Agglutination testing for human erythrocyte product in the rhesus macaque
- Source :
- Transfusion. 59:1518-1521
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- INTRODUCTION There has been interest in using human blood products in nonhuman primate models of trauma to supplement human studies and to provide evidence to guide novel trauma resuscitation strategies. The compatibility of human RBCs has not been extensively studied in nonhuman primate species. METHODS Whole blood samples were collected from five healthy, nontransfused, not previously pregnant Chinese-bred rhesus macaques. The whole blood was centrifuged, and the plasma was decanted from each sample. Group O-negative human RBCs were mixed with the plasma from the rhesus macaque monkeys. Compatibility testing was performed by an immediate spin test and polyspecific and monospecific anti-human globulin (AHG) tests in glass tubes. RESULTS Immediate spin testing revealed three out of five plasma samples (60%) from rhesus macaques caused at least 1+ agglutination with the human RBCs. Polyspecific anti-human globulin (AHG) tests demonstrated that two of five plasma samples (40%) from rhesus macaques caused at least 1+ agglutination with the human RBC, while the monospecific AHG testing revealed that the incompatibility was caused by C3d, not IgG. CONCLUSION Human RBCs are not compatible with the plasma of some, but not all, Chinese-bred rhesus macaques.
- Subjects :
- Erythrocytes
Globulin
Immunology
030204 cardiovascular system & hematology
Biology
ABO Blood-Group System
Andrology
03 medical and health sciences
0302 clinical medicine
Agglutination Tests
Animals
Humans
Immunology and Allergy
Whole blood
Plasma samples
Agglutination Testing
Human studies
Hemagglutination
Hematology
biology.organism_classification
Macaca mulatta
Nonhuman primate
Rhesus macaque
Immunoglobulin G
biology.protein
Female
Immediate spin
030215 immunology
Subjects
Details
- ISSN :
- 15372995 and 00411132
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Transfusion
- Accession number :
- edsair.doi.dedup.....6402cc3498b2dcd10c698ac3ff21c684