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In Silico Network Analysis of Ingredients of Cornus officinalis in Osteoporosis
- Source :
- Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
- Publication Year :
- 2021
- Publisher :
- International Scientific Literature, Inc., 2021.
-
Abstract
- BACKGROUND Cornus officinalis (CO), also known as 'Shanzhuyu', is one of the most common traditional Chinese herbs used against osteoporosis. Although previous studies have found that CO has beneficial effects in alleviating osteoporosis, its mechanisms remain unclear. MATERIAL AND METHODS In this study, we applied system bioinformatic approaches to investigate the possible therapeutic mechanisms of CO against osteoporosis. We collected the active ingredients of CO and their targets from the TCMSP, BATMAN-TCM, and ETCM databases. Next, we obtained the osteoporosis targets from differentially expressed mRNAs from the Gene Expression Omnibus (GEO) gene series (GSE35958). Next, the shared genes of the CO pharmacological targets and osteoporosis-related targets were selected to construct the protein-protein interaction network, based on the results from the STRING database. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out by using the clusterProfiler package in R software. RESULTS In all, there were 58 unique CO compounds and 518 therapeutic targets. Based on the GO and KEGG enrichment results of 98 common genes, we selected the top 25 terms, based on the terms' P values. We found that the anti-osteoporotic effect of CO may mostly involve the regulation of calcium metabolism and reactive oxygen species, and the estrogen signaling pathway and osteoclast differentiation pathway. CONCLUSIONS We found the possible mechanisms of CO in treating osteoporosis may be based on multiple targets and pathways. We also provided a theoretical basis and promising direction for investigating the exact anti-osteoporotic mechanisms of CO.
- Subjects :
- In silico
Osteoporosis
Osteoclasts
Computational biology
Cornus
Pharmacologic Actions
Interaction network
medicine
Humans
Computer Simulation
Protein Interaction Maps
KEGG
Medicine, Chinese Traditional
Gene
biology
Computational Biology
Cell Differentiation
Estrogens
General Medicine
Cornus officinalis
Chinese herbs
medicine.disease
biology.organism_classification
Molecular Docking Simulation
Gene Ontology
Estrogen signaling
Database Analysis
Reactive Oxygen Species
Drugs, Chinese Herbal
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 16433750 and 12341010
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
- Accession number :
- edsair.doi.dedup.....6402d6a2fea94a254fa867795ad90efc