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Fusobacterium nucleatum in biopsied tissues from colorectal cancer patients and alcohol consumption in Korea

Authors :
Ki Jong Rhee
Jung-Hyun Byun
Myungsook Kim
Kyungwon Lee
Tae Il Kim
Songyi Choi
Young Ah Kim
Jong Rak Choi
Seung-Tae Lee
Hyukmin Lee
Sun Sung Kwon
Source :
Scientific Reports, Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
Publication Year :
2020

Abstract

The roles of individual bacteria and their relationship in the development of colorectal cancer (CRC) remain unclear. We aimed to determine the prevalence of CRC-associated bacteria using quantitative real-time PCR (qPCR) or 16S rRNA analysis and the statistical correlations of patient demographics and clinical characteristics comprising alcohol consumption with CRC-associated bacteria. We determined the prevalence of five CRC-associated bacterial species in 38 CRC patients (39 samples) and 21 normal individuals using qPCR, and the relative abundance of bacterial taxa in the gut microbiome was assessed using 16S rRNA analysis. Fusobacterium nucleatum was the only bacterium that was significantly (P Fusobacterium, Peptostreptococcus, Collinsella, Prevotella, Parvimonas, and Gemella, in patients with CRC. An integrated analysis using 16S rRNA data and epidemiological characteristics showed that alcohol consumption was significantly correlated with the abundance of Fusobacterium OTUs. The correlation of alcohol consumption with the abundance of Fusobacterium OTUs in cancer tissue discovered using 16S rRNA analysis suggests a possible link between alcohol metabolism and subsequent tumorigenesis caused by F. nucleatum.

Details

ISSN :
20452322
Volume :
10
Issue :
1
Database :
OpenAIRE
Journal :
Scientific reports
Accession number :
edsair.doi.dedup.....64133f47dae655af3088aa913f6ac591