Back to Search
Start Over
Fusobacterium nucleatum in biopsied tissues from colorectal cancer patients and alcohol consumption in Korea
- Source :
- Scientific Reports, Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
- Publication Year :
- 2020
-
Abstract
- The roles of individual bacteria and their relationship in the development of colorectal cancer (CRC) remain unclear. We aimed to determine the prevalence of CRC-associated bacteria using quantitative real-time PCR (qPCR) or 16S rRNA analysis and the statistical correlations of patient demographics and clinical characteristics comprising alcohol consumption with CRC-associated bacteria. We determined the prevalence of five CRC-associated bacterial species in 38 CRC patients (39 samples) and 21 normal individuals using qPCR, and the relative abundance of bacterial taxa in the gut microbiome was assessed using 16S rRNA analysis. Fusobacterium nucleatum was the only bacterium that was significantly (P Fusobacterium, Peptostreptococcus, Collinsella, Prevotella, Parvimonas, and Gemella, in patients with CRC. An integrated analysis using 16S rRNA data and epidemiological characteristics showed that alcohol consumption was significantly correlated with the abundance of Fusobacterium OTUs. The correlation of alcohol consumption with the abundance of Fusobacterium OTUs in cancer tissue discovered using 16S rRNA analysis suggests a possible link between alcohol metabolism and subsequent tumorigenesis caused by F. nucleatum.
- Subjects :
- 0301 basic medicine
Male
Alcohol Drinking
Biopsy
030106 microbiology
lcsh:Medicine
Article
Microbiology
03 medical and health sciences
stomatognathic system
Republic of Korea
Prevotella
medicine
Humans
lcsh:Science
Clinical microbiology
Aged
Cancer
Multidisciplinary
biology
Fusobacterium nucleatum
Parvimonas
lcsh:R
Middle Aged
biology.organism_classification
16S ribosomal RNA
medicine.disease
Prognosis
Peptostreptococcus
Gastrointestinal Microbiome
stomatognathic diseases
030104 developmental biology
Fusobacterium
Case-Control Studies
Gemella
Fusobacterium Infections
lcsh:Q
Female
Colorectal Neoplasms
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....64133f47dae655af3088aa913f6ac591