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Increased leucocyte apoptosis in transfused β-thalassaemia patients

Authors :
Janet L. Kwiatkowski
Robert W. Grady
Daniele Alberti
Elliott Vichinsky
Annie Higa
Patricia J. Giardina
Patricia Evans
John B. Porter
Paul Harmatz
Felicia Trachtenberg
Thomas D. Coates
Bruce N. Ames
Patrick B. Walter
Ellen B. Fung
Ellis J. Neufeld
Nancy F. Olivieri
Source :
British Journal of Haematology. 160:399-403
Publication Year :
2012
Publisher :
Wiley, 2012.

Abstract

This exploratory study assessed apoptosis in peripheral blood leucocytes (PBL) from β-thalassaemia patients receiving chronic transfusions and chelation therapy (deferasirox or deferoxamine) at baseline, 1, 6, and 12 months. At baseline, thalassaemic PBLs presented 50% greater levels of Bax (BAX), 75% higher caspase-3/7, 48% higher caspase-8 and 88% higher caspase-9 activities and 428% more nucleosomal DNA fragmentation than control subjects. Only neutrophils correlated significantly with apoptotic markers. Previously, we showed that over the treatment year, hepatic iron declined; we now show that the ratio of Bax/Bcl-2 (BCL2), (-27·3%/year), and caspase-9 activity (-13·3%/year) declined in both treatment groups, suggesting that chelation decreases body iron and indicators of PBL apoptosis.

Details

ISSN :
00071048
Volume :
160
Database :
OpenAIRE
Journal :
British Journal of Haematology
Accession number :
edsair.doi.dedup.....64198173fd87503bf8af30a7270e0111
Full Text :
https://doi.org/10.1111/bjh.12076